Abstract: The invention is directed to a microfluidic unit (101) for isolating and culturing yeast cells. This microfluidic unit (101) comprises a medium inlet, a medium outlet, a passage interconnecting the inlet and outlet, and in the passage a single cell trapping chamber (109). Moreover, this microfluidic unit (101) comprises further in the passage a daughter cells trapping chamber (111), this chamber (111) is placed downstream of the single cell trapping chamber (109), and configured for retaining the daughter cells while allowing offspring of these daughter cells to escape with a flow of the medium in the passage.