Abstract: The present invention is directed to a method of monitoring the physiological state of a cell cultivation. Several parameters such as cell viability, growth, metabolic profile, and productivity may be monitored to establish a metabolic fingerprint or metabolomic profile of a cell culture.

Abstract: A method and system for analyzing a plurality of groups of time-series gene expressions including determining a time-dependent score that comprises a difference between the gene expression in the first group at a given time point and the gene expression in the second group at the given time point, determining an expected difference parameter that comprises a difference between a mean expression of the gene in a third group of samples and a mean expression of the gene in a fourth group of samples, created by randomly dividing the samples into two equal groups, comparing a difference between the time-dependent score and the expected difference parameter to a threshold at the given time point, determining the significant genes at each time point on the basis of the comparison.

Abstract: Metabolomic profiling of a biological sample using a separation-molecular ID process, such as gas chromatography-mass spectrometry (“GC-MS”), requires the derivatization of the original sample. Quantitative GC-MS metabolomics is possible if the derivative is in one-to-one proportional relationship with the original concentration profile, wherein the proportionality remaining constant among samples. Two types of biases may be introduced into determination of a metabolomic profile to alter these conditions. The first type of bias is produced by a change in the proportionality size between profiles and is corrected by way of an internal standard. The second type of bias may distort the one-to-one relationship and change the proportionality between the profiles to a different fold-extent for each metabolite in a sample. The metabolomic profile data is corrected from these biases to reduce the risk of assigning biological significance to changes due only to chemical kinetics.