Abstract: The present invention relates to fixed charge reagents and kits for use in tandem mass spectrometry methods involving multiplex analysis. The compounds of the invention are phenacylamide compounds. The invention also relates to methods for the quantification of for example peptides and proteins by tandem mass spectrometry techniques using said reagents and kits. The reagents and kits of the invention enable multiplexed analysis of several samples in one experiment.

Abstract: The present invention relates to a method of identifying a polypeptide using a class of novel, water-stable reagents. More specifically, the method according to the invention comprises the steps of (a) derivatization in an aqueous solution of the N-terminus of the polypeptide, or the N-termini of one or more peptides of the polypeptide, with at least one acidic reagent which comprises a sulfonyl moiety coupled to an activated acid moiety to provide one or more peptide derivatives; (b) analyzing at least one such derivative using a mass spectrometric technique to provide a fragmentation pattern; and (c) interpreting the fragmentation pattern. Furthermore, the present invention also relates to a kit for identifying a polypeptide by a mass spectrometric technique, which kit comprises at least one acidic reagent comprising a sulfonyl moiety coupled to an activated acid moiety in a container together with suitable means for analysis of the fragmentation pattern obtained.

Abstract: The present invention relates to a method of identifying a polypeptide, which method comprises the steps of (a) derivatization of the N-terminus of the polypeptide, or the N-termini of one or more peptides of the polypeptide, with at least one acidic reagent which comprises a sulfonyl moiety coupled to an activated acid moiety to provide one or more peptide derivatives; analyzing at least one such derivative using a mass spectrometric technique to provide a fragmentation pattern, and (c) interpreting the fragmentation pattern obtained, wherein the peptide or polypeptide is immobilized to a solid support at least during step(a). Furthermore, the present invention also relates to a kit for identifying a polypeptide by a mass spectrometric technique.

Abstract: The present invention relates to a method of identifying a polypeptide using a class of novel, water-stable reagents.