Abstract: One aspect of the present disclosure relates to an analyte sensor device. The analyte sensor device can include an optode layer that undergoes an optical change in the presence of an analyte. The analyte sensor device can also include a selectively-permeable membrane encapsulating the optode layer to form a stable membrane that that minimizes fouling of the analyte sensor device. The analyte sensor device can also include a plurality of microparticles that suppress a background physical interference on a detection of the optical change of the optode layer.

Abstract: One aspect of the present disclosure relates to an analyte sensor device. The analyte sensor device can include an optode layer that undergoes an optical change in the presence of an analyte. The analyte sensor device can also include a selectively-permeable membrane encapsulating the optode layer to form a stable membrane that that minimizes fouling of the analyte sensor device. The analyte sensor device can also include a plurality of microparticles that suppress a background physical interference on a detection of the optical change of the optode layer.

Abstract: An in vitro sensor point-of-care sensor including a substrate, a sensing system, and a reference system. The substrate can include a first cavity and a second cavity. The sensing system can be disposed within the first cavity and include an optode membrane, a selectively-permeable membrane, and a plurality of microbeads. The optode membrane can be sensitive to an analyte in the biological fluid. The selectively-permeable membrane can cover an opening of the first cavity. The plurality of microbeads can be associated with at least one of the optode membrane and the selectively-permeable membrane. The reference system can be disposed within the second cavity.

Abstract: An in vitro sensor for point-of-care detection of at least one analyte or reaction product includes an inert, impermeable substrate, a sensing system, and a reference system. The substrate includes a first transparent surface oppositely disposed from a second surface and first and second cavities. Each of the first and second cavities defines an opening at the second surface. The sensing system is disposed in at least a portion of the first cavity and includes an analyte-detection optode membrane, an analyte-permeable membrane, and a plurality of non-transparent microbeads associated with at least one of the analyte-detection optode membrane and the analyte-permeable membrane. The analyte-permeable membrane is layered upon the analyte-detection optode membrane and covers the opening of the first cavity. The reference system is disposed in at least a portion of the second cavity.

Abstract: An in vitro sensor for point-of-care detection of at least one analyte or reaction product includes an inert, impermeable substrate, a sensing system, and a reference system. The substrate includes a first transparent surface oppositely disposed from a second surface and first and second cavities. Each of the first and second cavities defines an opening at the second surface. The sensing system is disposed in at least a portion of the first cavity and includes an analyte-detection optode membrane, an analyte-permeable membrane, and a plurality of non-transparent microbeads associated with at least one of the analyte-detection optode membrane and the analyte-permeable membrane. The analyte-permeable membrane is layered upon the analyte-detection optode membrane and covers the opening of the first cavity. The reference system is disposed in at least a portion of the second cavity.