Abstract: An aspect of the present invention is the use of a preparation containing a phytepsin, more specifically a cyprosin, containing the heterodimer, its N-terminal pro-peptide, the mature N-terminal peptide, and mature C-terminal peptide, as well as other precursor species, processing products, and aggregate species, either isolated or in any combinations of the former, native, extracted and partially purified from flowers of Cynara cardunculus, or recombinant, extracted from the supernatant from a culture of Saccharomyces cereviseae genetically modified for the heterologous production of cyprosin, for therapeutic applications more precisely for its use as an antitumor agent.

Abstract: The invention relates to isolates DNA molecules encoding a protein for papaya fruit ripening, DNA constructs, host cells, and transgenic plants comprising the DNA molecules. The invention also relates to methods to promoting or relaying the fruit ripening of papaya plants through transformation of papaya with DNA construct containing the DNA molecules.

Abstract: The present invention relates to DNA constructs which include DNA molecules which affect papaya fruit ripening and DNA molecules which encode papaya ringspot virus coat protein. The present invention further relates to a method of controlling papaya fruit ripening while conferring resistance to Papaya Ringspot Virus by transforming plants with the DNA construct. The present invention also relates to expression systems, host cells, and transgenic plants containing the DNA constructs of the invention.

Abstract: This invention provides isolated and purified nucleotide sequences which are differentially expressed during pear fruit ripening, and their protein products. The isolated genes can be inserted into expresssion cassettes and cloned in an expression vector which can be used to transform a host cell by selected transformation methods. Transgenic plants can be regenerated from transformed plant cells by in vitro culture techniques. The nucleotide sequences disclosed in this invention encode proteins which are described as having an effective action in fruit ripening control. When used in antisense orientation they can delay fruit softening and mesocarp deterioration, bringing important advantages for fruit producers.

Abstract: The present invention relates to DNA constructs which include DNA molecules which affect papaya fruit ripening and DNA molecules which encode papaya ringspot virus coat protein. The present invention further relates to a method of controlling papaya fruit ripening while conferring resistance to Papaya Ringspot Virus by transforming plants with the DNA construct. The present invention also relates to expression systems, host cells, and transgenic plants containing the DNA constructs of the invention.

Abstract: The present invention relates to DNA molecules and DNA constructs which promote papaya fruit ripening. The present invention is also directed to methods for promoting or delaying the ripening of papaya plants through transformation of papaya with DNA constructs containing nucleic acids which encode proteins or polypeptides involved in papaya ripening. The invention also relates to expression systems, host cells, and plants containing such DNA constructs.