Abstract: The application provides devices, kits and methods for a streamlined lateral flow assay for sample preparation, optional amplification, and detection of a target nucleic acid using programmable nuclease reagents.

Abstract: A bioconjugate of an E. coli glucosylated O4 antigen polysaccharide covalently linked to a carrier protein and compositions thereof are provided. Also provided are recombinant host cells for producing the bioconjugate, and methods of producing the bioconjugate using the recombinant host cells. The recombinant host cells contain a nucleic acid encoding a glucosyl transferase capable of modifying the E. coli O4 antigen with glucose branching to produce the glucosylated O4 antigen polysaccharide. Bioconjugates of an E. coli glucosylated O4 antigen polysaccharide described herein can be used alone or in combination with one or more additional E. coli O-antigen polysaccharides to induce antibodies against an E. coli glucosylated antigen, and to vaccinate a subject against extra-intestinal pathogenic E. coli (ExPEC).

Abstract: A method can be used for manufacturing one or more bodies made of a porous material derived from precursors of the porous material in a sol-gel process. The method involves filling precursors of the porous material into a mold defining the shape of the body, where the precursors include at least two reactive components and a solvent, and forming a gel body. The step is then repeated so as to form several gel bodies. The gel bodies are then removed from the mold after a predetermined time in which the gel bodies are formed from the precursors of the porous material. The gel bodies are arranged adjacent to one another, a spacer is provided between two adjacent gel bodies so as to provide a clearance therebetween, and the solvent is then removed from the gel bodies.

Abstract: A mold for manufacturing a body made of a porous material derived from precursors of the porous material in a sol-gel process carried out within the mold can have a lower part defining an interior volume for receiving the precursors of the porous material, wherein the interior volume defines the shape of the body to be manufactured, and at least a first opening through which the body is removable from the lower part. Surfaces of the lower part facing the interior volume are at least partially provided with a coating made of a material that is electrically dissipative and non-sticky to a gel formed from the precursors of the porous material and/or the body.

Abstract: A method for manufacturing a body made of a porous material derived from precursors of the porous material in a sol-gel process, including (i) providing a mold, containing a lower part defining an interior volume for receiving the precursors of the porous material, wherein the lower part comprises a first opening, and surfaces of the lower part facing the interior volume are at least partially provided with a coating made of a material being electrically dissipative and non-sticky to the precursors of the porous material and/or the body, (ii) filling precursors of the porous material into the lower part in a first inert or ventilated region, wherein the precursors include two reactive components and a solvent, (iii) removing the body from the lower part through the first opening after a predetermined time, (iv) disposing the body onto a support, and (v) removing the solvent from the body.

Abstract: A work machine having a controller configured to receive a first position signal from a first boom position sensor, a second position signal from a second boom position sensor, and a load signal from the load measuring device, wherein the controller is further configured to calculate a map of hydraulic capacities within an envelope of movement for one or more of a first and a second actuators based on the first position signal, the second position signal, and the load signal, and generate a lift path of movement for the pin through at least a portion of the envelope based on the hydraulic capacities, wherein the movement envelope being smaller than the envelope.

Abstract: A work machine having an intelligent mechanical linkage performance system comprises a frame and a ground-engaging mechanism, a boom assembly coupled to the frame wherein the boom assembly comprises a large boom and a dipper stick. A load measuring device is coupled to the boom assembly and configured to generate a load signal indicative of a payload. A pin is coupled to the dipper stick wherein the pin has an envelope of movement throughout which the pin is moveable by the large boom and the dipper stick. A controller is configured to receive a first position signal from a first boom position sensor, a second position signal from a second boom position sensor, and the load signal from the load measuring device. The controller is further configured to calculate a map of hydraulic capacities within the envelope of movement.

Abstract: A work machine having a controller configured to receive a first position signal from a first boom position sensor, a second position signal from a second boom position sensor, and a load signal from the load measuring device, wherein the controller is further configured to calculate a map of hydraulic capacities within an envelope of movement for one or more of a first and a second actuators based on the first position signal, the second position signal, and the load signal, and generate a lift path of movement for the pin through at least a portion of the envelope based on the hydraulic capacities, wherein the movement envelope being smaller than the envelope.

Abstract: A process for purifying a polycarbodiimide comprising (a) providing a mixture comprising a polycarbodiimide and a carbodiimidization catalyst; (b) separating carbodiimidization catalyst from the polycarbodiimide by subjecting the mixture according to (a) to a first distillation, (c) adding an entrainer to the first bottom product obtained from (b) to obtain a mixture; (d) further separating carbodiimidization catalyst from the polycarbodiimide by subjecting the mixture obtained from (c) to a second distillation.

Abstract: Charging devices and methods for charging electrically powered vehicles are disclosed. One example charging device includes a processor configured to at least partially control a state voltage and a detection circuit coupled to the processor. The detection circuit includes an energy storage device and a discharge circuit coupled to the energy storage device. The energy storage device is configured to be charged by the state voltage. The discharge circuit is configured to discharge said energy storage device in response to a discharge command from said processor. The processor is configured to determine a charging state associated with the electrically powered vehicle based on a voltage across said energy storage device.

Abstract: A process for producing a polycarbodiimide, comprising polymerizing a diisocyanate in the presence of a carbodiimidization catalyst in a reaction vessel in liquid phase at a temperature in the range of from 20 to 250° C., at a pressure in the range of from 20 to 800 mbar and in the presence of at least one inert gas, wherein the at least one inert gas is introduced into the liquid phase in the reaction vessel with a flow rate in the range of from 0.1 x/h to 100 x/h, x being the volume of the reaction vessel.

Abstract: A process for producing a polycarbodiimide, comprising polymerizing a diisocyanate in the presence of a carbodiimidization catalyst in a reaction vessel in liquid phase at a temperature in the range of from 20 to 250° C., at a pressure in the range of from 20 to 800 mbar and in the presence of at least one inert gas, wherein the at least one inert gas is introduced into the liquid phase in the reaction vessel with a flow rate in the range of from 0.1 x/h to 100 x/h, x being the volume of the reaction vessel.

Abstract: A process for purifying a polycarbodiimide comprising (a) providing a mixture comprising a polycarbodiimide and a carbodiimidization catalyst; (b) separating carbodiimidization catalyst from the polycarbodiimide by subjecting the mixture according to (a) to a first distillation, (c) adding an entrainer to the first bottom product obtained from (b) to obtain a mixture; (d) further separating carbodiimidization catalyst from the polycarbodiimide by subjecting the mixture obtained from (c) to a second distillation.

Abstract: A charging device for charging an energy storage device is described. The charging device includes a memory for storing a plurality of state machines and a processing device coupled to the memory. The processing device is configured to select a state machine of the plurality of state machines and to operate the charging device in accordance with the selected state machine.

Abstract: The invention relates to a process for the production of composites, composed of at least one outer layer b) and of an isocyanate-based rigid foam a), where the outer layer b) is moved continuously and the starting material for the isocyanate-based rigid foam a) is applied to the outer layer b), which comprises achieving the application of the liquid starting material for the isocyanate-based rigid foam a) by means of at least one fixed tube c) which has openings f) and which has been placed, with respect to the outer layer b), so as to be parallel to the plane of the outer layer and at right angles to the direction of movement.

Abstract: A charging device for a power storage device includes a power conduit configured to deliver current to the power storage device and a detection system configured to be coupled to the power conduit. The detection system includes a current control device coupled to the power conduit and configured to control the current delivered to the power storage device. The detection system also includes a test conductor, a current sensor coupled to the test conductor, and a controller coupled to the current sensor by the test conductor. The current sensor is arranged to detect an amount of current within the test conductor and configured to generate a current measurement signal representative of the current detected. The controller is configured to generate a test signal, transmit the test signal through the test conductor, receive the current measurement signal, and determine a status of the detection system based on the current measurement signal.

Abstract: A method of assembling an electric drive system includes providing a power conversion assembly and coupling an electric power source and a motor to the power conversion assembly. The method also includes coupling a computing device that includes a processor and a memory device operatively coupled to the processor to the power conversion assembly. The method further includes configuring the computing device to record at least one measurement related to a speed of the motor. The method also includes configuring the computing device to calculate a power output limit of the power conversion assembly and limit the power output of the power conversion assembly by limiting torque induced by the motor as a function of the speed of the motor.

Abstract: The invention relates to a process for the production of composites, composed of at least one outer layer b) and of an isocyanate-based rigid foam a), where the outer layer b) is moved continuously and the starting material for the isocyanate-based rigid foam a) is applied to the outer layer b), which comprises achieving the application of the liquid starting material for the isocyanate-based rigid foam a) by means of at least one fixed tube c) which has openings f) and which has been placed, with respect to the outer layer b), so as to be parallel to the plane of the outer layer and at right angles to the direction of movement.

Abstract: The invention relates to a process for the production of composites, composed of at least one outer layer b) and of an isocyanate-based rigid foam a), where the outer layer b) is moved continuously and the starting material for the isocyanate-based rigid foam a) is applied to the outer layer b), which comprises achieving the application of the liquid starting material for the isocyanate-based rigid foam a) by means of at least one fixed tube c) which has openings f) and which has been placed, with respect to the outer layer b), so as to be parallel to the plane of the outer layer and at right angles to the direction of movement.