Abstract: Embodiments of the present invention generally relate to recombinant alpha 1-antitrypsin (AAT) proteins, including variants of human AAT with individually introduced mutations, compositions containing such recombinant AAT proteins and carriers, expression plasmids or vectors and host cells that express such recombinant AAT proteins, methods of producing such recombinant AAT proteins, and methods of treating AAT deficiency-related diseases, disorders, and conditions or diseases, disorders, and conditions resulting in protease-induced tissue damage in a subject in need thereof with the recombinant AAT proteins and/or recombinant AAT protein compositions described here. The recombinant AAT proteins derived from mammalian host cells as produced by the methods described here may be produced in large quantities, without any animal components, i.e., highly pure, highly glycosylated, and may be advantageously used over plasma-derived AAT.

Abstract: Recombinant proteins are of great commercial interest. Yet, most current production methods in mammalian cells involve the time- and labor-consuming step of creating stable cell lines. Production methods based on transient gene expression are advantageous in terms of speed and versatility, yet, thus far, those methods have not shown the specific productivity, batch yield and volumetric yield to be an economic alternative to stable cell lines. The inventors improved on the methodology of transient transfection and achieved commercially relevant yields in terms of specific productivity (exceeding 35 pg per cell per day), batch yield (exceeding 700 mg/l) and volumetric yield.

Abstract: Fluorescent protein expressing cells with very high fluorescence are described as well as a method to obtain such cells. The fluorescence of such cells can be as high as about 1000 times the auto-fluorescence of the respective host cell. Due to the high fluorescence, said cells are very suitable tools in the development, surveillance and/or optimization of production processes, for selection of culture media and process parameters and for the determination of cell growth and the detection, quantification and monitoring of biomass.

Abstract: Fluorescent protein expressing cells with very high fluorescence are described as well as a method to obtain such cells. The fluorescence of such cells can be as high as about 1000 times the auto-fluorescence of the respective host cell.

Abstract: A static memory is constructed in a plurality of hierarchy levels. Beneficial realization possibilities are set forth with respect to the surface utilization for the drive and read-out circuits in the second hierarchy level which are especially critical. Memory cells that supply a strong cell signal are advantageously utilized so that a low expense is needed in the read circuit. By displacing periphery circuits into higher hierarchy levels, a short access time and a reduced surface requirement arise.

Abstract: Static memory having pipeline registers. The static memory has a plurality of hierarchy levels connected by pipeline registers. This architecture is very beneficial since the area requirements for the drive and read-out circuits in the first hierarchy level are especially critical. Advantageously, memory cells are used which have write and read word lines as well as separate write and read data lines and which also supply a strong cell signal so that only a few components are needed for the read circuit. A new clock format with an arrangement of pipeline registers is proposed for the appertaining memory for which power consumption is reduced by disconnecting the clocks in the lower hierarchy levels, resulting in increased area savings.

Abstract: Memory cells are disclosed that avoid the utilization of analog circuits in the memory peripheral circuits when they are utilized in static memory modules and that intended to enhance the disturbed reliability when confronted by technology modifications and parameter fluctuations. Write-in thereby occurs from a write data line via a write selection transistor and read-out occurs via a read selection transistor onto a read data line. A second inverter formed of two field effect transistors serves as a feedback element in order to statically maintain the cell information. Due to an implemented asymmetry in the dimensioning between the first and second inverters, the memory cell is significantly less susceptible to information loss upon read-out when compared to a heretofore known memory cell. A precharging of the read data line is not required with these memory cells.