Patents by Inventor Marian Rehak
Marian Rehak has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11919001Abstract: This invention generally relates to methods and systems for dispensing a droplet, in particular a picolitre volume droplet containing one or more entities.Type: GrantFiled: June 21, 2018Date of Patent: March 5, 2024Assignee: SPHERE FLUIDICS LIMITEDInventors: Dimitris Josephides, Marian Rehak, David Holmes, Frank F. Craig
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Publication number: 20210308682Abstract: We describe instruments, and cartridges therefor, for processing droplets of water in oil-in-water emulsions, the droplets containing biological entities such as cells. In one embodiment a method of such processing comprises: providing a plurality of the entities in a fluid: preparing a droplet from said fluid: determining whether said droplet contains one or more entities of said plurality of entities, or whether said droplet does not contain a said entity; sorting said droplet dependent on an outcome of said determination; and dispensing said sorted droplet into a reservoir, wherein said dispensing comprises: identifying said sorted droplet for dispensing; extracting said sorted droplet from a first fluidic flow path of said fluid by transferring said sorted droplet from said first fluidic flow path into a second fluidic flow path; and ejecting said sorted droplet from said second fluidic flow path into said reservoir by applying pressure to said second fluidic flow path.Type: ApplicationFiled: June 16, 2021Publication date: October 7, 2021Inventors: Frank F. CRAIG, Marian Rehak, David Holmes, Clive A. Smith, Xin Liu, Giuseppe Benazzi, Xin Li, Vinayaka Pawate, Robert Frank Marchington, Richard Francis Day, Michael Stewart Hazell
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Publication number: 20210293691Abstract: A method of processing droplets in a microfluidic system. The method may comprise capturing a time sequence of images of a droplet as it passes through a channel in a microfluidic system. The method may further comprise processing each image of the sequence of images using a convolutional neural network to count a number of cells or other entities visible in each image the droplet. The method may further comprise processing the count of the number of cells or other entities visible in each image of the droplet to determine an estimated number of cells or other entities in the droplet. The method/system may further comprise controlling a microfluidic process performed on the droplet responsive to the estimated number of cells or other entities in the droplet. Implementations of the method use the changing orientation and disposition of droplet contents in combination with machine learning to improve monoclonality assurance.Type: ApplicationFiled: August 6, 2019Publication date: September 23, 2021Inventors: Nicholas Dayrell-Armes, David Holmes, Frank F Craig, Marian Rehak, Dimitris Josephides, Robert Salter, William Whitley, Sinan Gokkaya, Raphael Ruis
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Patent number: 11077441Abstract: Instruments and cartridges for processing droplets in emulsions containing biological entities such as cells. A method of such processing comprises providing a plurality of the entities in a fluid; preparing a droplet from the fluid; determining whether the droplet contains one or more entities of said plurality of entities, or whether said droplet does not contain a said entity; sorting said droplet dependent on an outcome of the determination; and dispensing the sorted droplet into a reservoir. The dispensing may comprise identifying and extracting the sorted droplet from a first fluidic flow path of said fluid by transferring the sorted droplet from into a second fluidic flow path and then ejecting the sorted droplet into a reservoir by applying pressure to the second fluidic flow path. The droplet contents may be tracked so that the contents of an individual droplet can be sorted, selectively dispensed, and retrieved.Type: GrantFiled: June 3, 2016Date of Patent: August 3, 2021Assignee: Sphere Fluidics LimitedInventors: Frank F. Craig, Marian Rehak, David Holmes, Clive A. Smith, Xin Liu, Giuseppe Benazzi, Xin Li, Vinayaka Pawate, Robert Frank Marchington, Richard Francis Day, Michael Stuart Hazell
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Publication number: 20200330994Abstract: A method of performing a biological reaction in a microfluidic droplet, and in particular to a method of performing genome editing in a microfluidic droplet. The invention also relates to microfluidic systems and instrumentations or products for performing these reactions.Type: ApplicationFiled: December 19, 2018Publication date: October 22, 2020Inventors: Frank F. Craig, Serena A. Davoli, Frank Gesellchen, Xin Liu, Marian Rehak, Elena Shvets
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Publication number: 20200215546Abstract: This invention generally relates to methods and systems for dispensing a droplet, in particular a picolitre volume droplet containing one or more entities.Type: ApplicationFiled: June 21, 2018Publication date: July 9, 2020Inventors: Dimitris JOSEPHIDES, Marian REHAK, David HOLMES, Frank F. Craig
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Publication number: 20180133715Abstract: Instruments and cartridges for processing droplets in emulsions containing biological entities such as cells. A method of such processing comprises providing a plurality of the entities in a fluid; preparing a droplet from the fluid; determining whether the droplet contains one or more entities of said plurality of entities, or whether said droplet does not contain a said entity; sorting said droplet dependent on an outcome of the determination; and dispensing the sorted droplet into a reservoir. The dispensing may comprise identifying and extracting the sorted droplet from a first fluidic flow path of said fluid by transferring the sorted droplet from into a second fluidic flow path and then ejecting the sorted droplet into a reservoir by applying pressure to the second fluidic flow path. The droplet contents may be tracked so that the contents of an individual droplet can be sorted, selectively dispensed, and retrieved.Type: ApplicationFiled: June 3, 2016Publication date: May 17, 2018Inventors: Frank F. CRAIG, Marian REHAK, David HOLMES, Clive A. SMITH, Xin LIU, Giuseppe BENAZZI, Xin LI, Vinayaka PAWATE
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Patent number: 9678070Abstract: The invention provides an apparatus and methods for the electrochemical detection and/or quantitation of an analyte in a sample, wherein the device comprises a substrate and a detector, wherein the substrate comprises a labeled binding agent, wherein the label is an enteric material particle that encapsulates a ferrocene methanol redox species.Type: GrantFiled: April 29, 2015Date of Patent: June 13, 2017Assignee: Church & Dwight Co., Inc.Inventors: Albert R. Nazareth, Shang Li, Giles H. W. Sanders, Anthony Edward George Cass, Marian Rehak
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Publication number: 20160320383Abstract: The invention provides an apparatus and methods for the electrochemical detection and/or quantitation of an analyte in a sample, wherein the device comprises a substrate and a detector, wherein the substrate comprises a labeled binding agent, wherein the label is an enteric material particle that encapsulates a ferrocene methanol redox species.Type: ApplicationFiled: April 29, 2015Publication date: November 3, 2016Applicant: Church & Dwight, Co., Inc.Inventors: Albert R. Nazareth, Shang Li, Giles H.W. Sanders, Anthony Edward George Cass, Marian Rehak
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Publication number: 20140248642Abstract: The invention provides apparatus for the quantitative analysis of an analyte in a sample, comprising (i) a solid phase; and (ii) a detector, wherein the surface of the solid phase comprises (a)a first position for sample application, and (b)a second position, distant from the first position, wherein a first molecule that binds to the analyte and is capable of releasing a detectable species is either deposited at the first position or is added to the sample prior to application to the LF membrane, and wherein a second molecule that binds to the analyte is immobilised at the second position, and wherein an enzyme is immobilised, co-located with the immobilised molecule at the second position, and wherein the detector is located in close proximity to the immobilised molecule at the second position.Type: ApplicationFiled: July 19, 2012Publication date: September 4, 2014Applicant: BIO NANO CONSULTINGInventors: Anthony Edward George Cass, Almudena Celaya Sanfiz, Marian Rehak