Abstract: The present disclosure provides liposomal compositions comprising lipids, in particular phospholipids and cholesterol, a negatively charged biomolecule, such as a polynucleotide, an ionizable aminoglycoside, such as chitosan, and an oil-based carrier. The compositions can be used for delivery of the biomolecule to targeted cells. The disclosure also provides the use of the composition for the treatment or prevention of cancer or infectious disease or ailment ameliorated by humoral and cellular immune response. The compositions can also be used for expressing polypeptides encoded by the nucleic acid components in the targeted cells.

Abstract: Provided herein are methods and compositions for the delivery of at least two active, therapeutic, or pharmaceutical agents in an oil-in-water emulsion, wherein at least one agent is delivered in the hydrophobic phase of the emulsion and at least one agent is delivered in the aqueous phase of the emulsion.

Abstract: The present disclosure provides methods, uses and compositions and kits for use in inducing an antibody immune response in a human subject. The methods and uses involve administering parenterally a low dose volume of a composition comprising an antigen comprising a B-cell epitope, an amphipathic compound, and a hydrophobic carrier, wherein the low dose volume of the composition is less than 100 ?l and induces an antibody immune response to the B-cell epitope in the human subject.

Abstract: The present disclosure provides methods, uses and compositions and kits for use in inducing an antibody immune response in a human subject. The methods and uses involve administering parenterally a low dose volume of a composition comprising an antigen comprising a B-cell epitope, an amphipathic compound, and a hydrophobic carrier, wherein the low dose volume of the composition is less than 100 ?l and induces an antibody immune response to the B-cell epitope in the human subject.

Abstract: The present application relates to vaccine compositions comprising an amphipathic compound, a neoantigen and a hydrophobic carrier. Further described are methods and use of the vaccine composition for inducing an antibody immune response and/or a cell-mediated immune response to the neoantigen, as well as methods and uses of the vaccine compositions in the treatment of cancer.

Abstract: The present application relates generally to methods for treating tumors, and in particular to methods for improving the efficacy of a survivin therapeutic in the treatment of tumors by improving survivin specific T cell infiltration in tumor.

Abstract: The present application relates to vaccine compositions comprising an amphipathic compound, a neoantigen and a hydrophobic carrier. Further described are methods and use of the vaccine composition for inducing an antibody immune response and/or a cell-mediated immune response to the neoantigen, as well as methods and uses of the vaccine compositions in the treatment of cancer.

Abstract: The present disclosure relates to the targeted delivery of defined active agents and/or immunomodulatory agents to lymph nodes or lipoid cells in a lymphatic tissue. More particularly, the invention provides methods for targeted delivery of a defined active agent or immunomodulatory agent to lymph nodes or lymphoid cells in a lymphatic tissue, comprising administering to a subject in need thereof a composition comprising the active agent or immunomodulatory agent, one or more lipid-based structures, and a hydrophobic carrier.

Abstract: The present disclosure relates to methods for preparing a dried preparation comprising lipids and therapeutic agents whereby the therapeutic agents are incorporated both before and after sizing of lipid vesicle particles to a mean particle size of ?120 nm and a polydispersity index (PDI) of ?0.1. The present application also provides stable, water-free pharmaceutical compositions comprising one or more lipid-based structures having a single layer lipid assembly, at least two therapeutic agents, and a hydrophobic carrier, as well as methods of treatment, uses and kits relating thereto, such as for example for inducing an antibody and/or CTL immune response.

Abstract: The present disclosure relates to methods for preparing a dried preparation comprising lipids and a therapeutic agent by using lipid vesicle particles having a mean particle size of ?120 nm and a polydispersity index (PDI) of ?0.1. The present application also provides stable, water-free pharmaceutical compositions comprising one or more lipid-based structures having a single layer lipid assembly, at least one therapeutic agent, and a hydrophobic carrier, as well as methods of treatment, uses and kits relating thereto, such as for example for inducing an antibody and/or CTL immune response.

Abstract: The present disclosure provides methods, uses and compositions and kits for use in inducing an antibody immune response in a human subject. The methods and uses involve administering parenterally a low dose volume of a composition comprising an antigen comprising a B-cell epitope, an amphipathic compound, and a hydrophobic carrier, wherein the low dose volume of the composition is less than 100 ?l and induces an antibody immune response to the B-cell epitope in the human subject.

Abstract: The present application relates to vaccine compositions comprising an amphipathic compound, a neoantigen and a hydrophobic carrier. Further described are methods and use of the vaccine composition for inducing an antibody immune response and/or a cell-mediated immune response to the neoantigen, as well as methods and uses of the vaccine compositions in the treatment of cancer.

Abstract: The present disclosure provides methods, vaccines and kits for potentiating an immune response to an antigen in a subject. The methods comprise administering to the subject at least one dose of a depot-forming vaccine comprising one or more antigens in a hydrophobic carrier; and subsequently administering to the subject at least one dose of a non-depot-forming vaccine comprising the one or more antigens.

Abstract: The present invention relates to therapeutic use of a combination of Myxoma virus, including in combination with rapamycin. Treatment with rapamycin enhances the ability of Myxoma virus to selectively infect cells that have a deficient innate anti-viral response, including cells that are not responsive to interferon. The combination of rapamycin and Myxoma virus can be used to treat diseases characterized by the presence of such cells, including cancer. The invention also relates to therapeutic use of Myxoma virus that does not express functional M135R.

Abstract: The present invention relates to therapeutic use of a combination of Myxoma virus, including in combination with rapamycin. Treatment with rapamycin enhances the ability of Myxoma virus to selectively infect cells that have a deficient innate anti-viral response, including cells that are not responsive to interferon. The combination of rapamycin and Myxoma virus can be used to treat diseases characterized by the presence of such cells, including cancer. The invention also relates to therapeutic use of Myxoma virus that does not express functional M135R.

Abstract: The present invention relates to therapeutic use of a combination of Myxoma virus, including in combination with rapamycin. Treatment with rapamycin enhances the ability of Myxoma virus to selectively infect cells that have a deficient innate anti-viral response, including cells that are not responsive to interferon. The combination of rapamycin and Myxoma virus can be used to treat diseases characterized by the presence of such cells, including cancer. The invention also relates to therapeutic use of Myxoma virus that does not express functional M135R.

Abstract: The present invention relates to therapeutic use of a combination of Myxoma virus, including in combination with rapamycin. Treatment with rapamycin enhances the ability of Myxoma virus to selectively infect cells that have a deficient innate anti-viral response, including cells that are not responsive to interferon. The combination of rapamycin and Myxoma virus can be used to treat diseases characterized by the presence of such cells, including cancer. The invention also relates to therapeutic use of Myxoma virus that does not express functional M135R.