Abstract: A microfluidic component used for measuring electrical impedance across a biological object, the component including a microfluidic space including a zone referred to as measurement zone, at least two electrodes arranged facing one another on each side of the measurement zone, the component being formed by assembling, along a longitudinal junction plane, at least two superposed layers referred to as lower layer and upper layer, the two layers each having at least one cavity, the two layers being assembled with one another in such a way as to position the two cavities facing one another in order to form the microfluidic space.

Abstract: The invention relates to a device for transiently contacting at least one unit for capturing biological targets with a body fluid containing them, a method for recovering the captured targets for analysis, and a system for contacting and recovering a capture substrate included in said unit. The invention relates to samples obtained in particular in vivo from body fluids of the human body, e.g. circulating body fluids, said fluids possibly containing, as targets, proteins, oligonucleotides such as RNA or DNA, antibodies, enzymes or cells.

Abstract: A method of sampling biological or biochemical species, comprising the following steps: a) arranging a capture surface (SC) for said biological or biochemical species in contact with a biological tissue or fluid (TB); and b) rinsing said surface to remove biological or biochemical species that have not been adsorbed; the method being characterised in that said capture surface is the surface of a nanoporous material (MC). A method of analysing said biological or biochemical species, characterised by the use of said surface as an analysis support. The analysis may in particular be performed using a method selected from mass spectroscopy with laser desorption and fluorescence imaging.

Abstract: The invention relates to a sampling device adapted to be inserted into a hollow tubular endpiece of the needle or catheter type, and to emerge from the endpiece with a view to contact with a bodily fluid containing biological samples to be sampled, to a sampling system incorporating this endpiece and this device, which is mounted so as to slide in the latter, and to a method for manufacturing this device. This sampling device comprises a framework (5) microstructured by openings (5b), and a biocompatible and porous crosslinked polymer layer which comprises capture supports adapted to capture the said targets and which is adapted to retain these supports from the fluid and to let through only fluid particles including these targets with a size of less than a cutoff size, the said polymer layer filling all or some of the said openings, so as to be retained by the said framework.

Abstract: The present invention relates to a method for assaying catalytic plasma enzymes, such as transaminases and NADH-dependent enzymes, in a sample of whole blood, by measuring, in a microfluidic chamber, the decrease in the fluorescence of NADH consumed during the enzymatic reactions catalyzed by the NADH-dependent enzymes.

Abstract: The invention relates to a device for transiently contacting at least one unit for capturing biological targets with a body fluid containing them, a method for recovering the captured targets for analysis, and a system for contacting and recovering a capture substrate included in said unit. The invention relates to samples obtained in particular in vivo from body fluids of the human body, e.g. circulating body fluids, said fluids possibly containing, as targets, proteins, oligonucleotides such as RNA or DNA, antibodies, enzymes or cells.

Abstract: A device (6) to sample molecules of biological interest is described. The device (6) includes capture zones (10) whose developed surface area is much greater than the surface so that sampling by contact allows a sufficient quantity of molecules to be obtained to achieve reliable analysis, whilst being sized so that the device (6) is neither invasive nor aggressive. In particular, the device comprises tiered capture zones (10) located on a support (12) fabricated using microtechnology techniques; the capture zones (10) may advantageously be functionalised.

Abstract: The present invention relates to a method for assaying catalytic plasma enzymes, such as transaminases and NADH-dependent enzymes, in a sample of whole blood, by measuring, in a microfluidic chamber, the decrease in the fluorescence of NADH consumed during the enzymatic reactions catalyzed by the NADH-dependent enzymes.

Abstract: A device (6) to sample molecules of biological interest is described. The device (6) includes capture zones (10) whose developed surface area is much greater than the surface so that sampling by contact allows a sufficient quantity of molecules to be obtained to achieve reliable analysis, whilst being sized so that the device (6) is neither invasive nor aggressive. In particular, the device comprises tiered capture zones (10) located on a support (12) fabricated using microtechnology techniques; the capture zones (10) may advantageously be functionalised.

Abstract: A microtechnological device (6) for sampling cells on or in a tissue or other element of the body. It comprises a support (12) having at least one surface of interest (14) on which is present at least one recovery zone (10) comprised of a bottom wall (22) equipped with a plurality of protuberances (24). Preferably, the microtechnological device comprises at least one recovery zone covered with a coating that has the power to attract the cells to be sampled.