Abstract: Disclosed is a method for adjusting the amplification efficiency of a target polynucleotide in the amplification of the target polynucleotide by PCR using primers (i) to (iii) below, the method comprising adjusting the amplification efficiency of the target polynucleotide by changing the quantity ratio of the primers (i) to (iii) below: (i) a first primer which is able to be base-paired with the target polynucleotide; (ii) a second primer which is able to be base-paired with the target polynucleotide in competition with the first primer and from which extension reaction by PCR less occurs as compared to the first primer; and (iii) a third primer designed to allow for the amplification of the target polynucleotide in pairs with the first primer.

Abstract: The present invention provides a polymorphism detection probe that can identify a different polymorphism in a K-ras gene easily with high reliability and use of the polymorphism detection probe.

Abstract: The present invention provides a dry reagent capable of amplifying a nucleic acid even after being stored in a dry state at room temperature, a dry reagent kit, a reagent container, and a method for producing the dry reagent and methods of producing and using thereof.

Abstract: A probe for detecting a polymorphism at position ?1639 of the VKORC1 gene, the probe comprising an oligonucleotide having a nucleotide sequence having a length of 10 to 50 nucleotides, which nucleotide sequence comprises the nucleotides 80 to 89 of SEQ ID NO:1 or 2 and has a homology to SEQ ID NO:1 or 2 except that the nucleotide corresponding to the nucleotide at position 80 in SEQ ID NO:1 or 2 is cytosine, which nucleotide corresponding to the nucleotide at position 80 is labeled with a fluorescent dye.

Abstract: The present disclosure provides probes for detecting a polymorphism in the PON1 gene.

Abstract: The present invention provides a dry reagent capable of amplifying a nucleic acid even after being stored in a dry state at room temperature, a dry reagent kit, a reagent container, and a method for producing the dry reagent and methods of producing and using thereof.

Abstract: The present invention provides probes that can identify different polymorphisms in a c-kit gene easily with high reliability and use thereof.

Abstract: The present invention provides a polymorphism detection probe that can identify a different polymorphism in a K-ras gene easily with high reliability and use of the polymorphism detection probe.

Abstract: The present invention provides a probe that can identify a polymorphism in an MPL gene easily and with high reliability and use of the probe. Used as the probe for detecting a polymorphism in the MPL gene is a probe containing any one of oligonucleotides (P1), (P1?), (P2), and (P2?), wherein: (P1) is a 9- to 50-mer oligonucleotide composed of a base sequence including 11535th to 11543rd bases in SEQ ID NO: 1 and having the 11543rd base in its 3? end region; (P1?) is an oligonucleotide composed of a base sequence complementary to the oligonucleotide (P1); (P2) is a 10- to 50-mer oligonucleotide composed of a base sequence including 11535th to 11544th bases in SEQ ID NO: 1 and having the 11544th base in its 3? end region; and (P2?) is an oligonucleotide composed of a base sequence complementary to the oligonucleotide (P2).

Abstract: Disclosed is a method for adjusting the amplification efficiency of a target polynucleotide in the amplification of the target polynucleotide by PCR using primers (i) to (iii) below, the method comprising adjusting the amplification efficiency of the target polynucleotide by changing the quantity ratio of the primers (i) to (iii) below: (i) a first primer which is able to be base-paired with the target polynucleotide; (ii) a second primer which is able to be base-paired with the target polynucleotide in competition with the first primer and from which extension reaction by PCR less occurs as compared to the first primer; and (iii) a third primer designed to allow for the amplification of the target polynucleotide in pairs with the first primer.

Abstract: A probe for detecting a polymorphism in exon 12 of the JAK2 gene, the probe comprising at least one of the oligonucleotides in (a) to (c) below: (a) an oligonucleotide having a nucleotide sequence homologous to the sequence complementary to a nucleotide sequence having a length of 19 to 50 nucleotides containing the nucleotides 114 to 132 of SEQ ID NO:1; (b) an oligonucleotide having a nucleotide sequence homologous to the sequence complementary to a nucleotide sequence having a length of 22 to 50 nucleotides containing the nucleotides 122 to 143 of SEQ ID NO:1; and (c) an oligonucleotide having a nucleotide sequence homologous to a nucleotide sequence having a length of 10 to 50 nucleotides containing the nucleotides 41 to 50 of SEQ ID NO:11.

Abstract: A nucleic acid abundance ratio measurement device includes a detection section that detects a detection signal over different temperature ranges of a melting curve for a nucleic acid mixture having one or more melting temperatures, and an abundance ratio computation section that computes a nucleic acid abundance ratio based on a ratio of characteristic amounts obtained from the detection signal detected by the detection section and based on detection amount data.

Abstract: A probe for detecting a polymorphism at position ?1639 of the VKORC1 gene, the probe comprising an oligonucleotide having a nucleotide sequence having a length of 10 to 50 nucleotides, which nucleotide sequence comprises the nucleotides 80 to 89 of SEQ ID NO:1 or 2 and has a homology to SEQ ID NO:1 or 2 except that the nucleotide corresponding to the nucleotide at position 80 in SEQ ID NO:1 or 2 is cytosine, which nucleotide corresponding to the nucleotide at position 80 is labeled with a fluorescent dye.