Abstract: Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), expoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT), Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside.

Abstract: Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), epoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT), Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside.

Abstract: The invention relates to novel tomato plants producing fruits displaying a modified sugar content, particularly displaying an increased sucrose content. The invention also relates to seeds and parts of said plants, for example fruits. The invention further relates to methods of making and using such seeds and plants. The invention also relates to a sucrose modifier SucMod allele, which, when combined with a sucrose accumulation TIV allele derived from a green-fruited wild tomato accession, significantly alters the proportion of sugar stored in the fruit, confers increased fruit sucrose content at the expense of hexose sugars and results in a fruit with a distinctive flavor.

Abstract: Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), epoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT), Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside.

Abstract: The present invention relates to novel tomato plants producing fruits displaying a modified sugar content, particularly displaying an increased sucrose content. The present invention also relates to seeds and parts of said plants, for example fruits. The present invention further relates to methods of making and using such seeds and plants. The present invention also relates to a novel sucrose modifier SucMod allele, which, when combined with a sucrose accumulation TIV allele derived from a green-fruited wild tomato accession, significantly alters the proportion of sugar stored in the fruit, confers increased fruit sucrose content at the expense of hexose sugars and results in a fruit with a distinctive flavour.

Abstract: Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), expoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT). Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside.

Abstract: A method of increasing biomass, vigor and/or yield of a plant is disclosed. The method comprises expressing within the plant an exogenous polypeptide comprising a UGGPase activity. The polypeptide may comprise an amino acid sequence at least 90% homologous, and/or at least 80% identical to SEQ ID NO: 33 as determined using the BlastP software of the National Center of Biotechnology Information (NCBI) using default parameters. Polynucleotides encoding same and plants expressing same are also disclosed.

Abstract: A method of increasing biomass, vigor and/or yield of a plant is disclosed. The method comprises expressing within the plant an exogenous polypeptide comprising a UGGPase activity. The polypeptide may comprise an amino acid sequence at least 90% homologous, and/or at least 80% identical to SEQ ID NO: 33 as determined using the BlastP software of the National Center of Biotechnology Information (NCBI) using default parameters. Polynucleotides encoding same and plants expressing same are also disclosed.

Abstract: The present invention provides a method of identifying alkaline-?-galactosidases. Also provided are polynucleotide sequences encoding polypeptides having an alkaline-?-galactosidase activity, oligonucleotides and oligonucleotide analogs derived from the polynucleotide sequences, peptides and peptide analogues, antibodies recognizing same and methods of using same.

Abstract: A method of increasing biomass, vigor and/or yield of a plant is disclosed. The method comprises expressing within the plant an exogenous polypeptide comprising a UGGPase activity. The polypeptide may comprise an amino acid sequence at least 90% homologous, and/or at least 80% identical to SEQ ID NO: 33 as determined using the BlastP software of the National Center of Biotechnology Information (NCBI) using default parameters. Polynucleotides encoding same and plants expressing same are also disclosed.

Abstract: The present invention provides a method of identifying alkaline-?-galactosidases. Also provided are novel polynucleotide sequences encoding polypeptides having an alkaline-?-galactosidase activity, oligonucleotides and oligonucleotide analogs derived from the polynucleotide sequences, peptides and peptide analogues, antibodies recognizing same and methods of using same.

Abstract: A method for controlling starch synthesis in tomatoes including providing a population of plants derived from interspecific crosses of Lycopersicon spp. with Lycopersicon esculentum genotypes, and selecting individuals of the population that each contain an allele of a gene that increases starch synthesis, the gene originating from the Lycopersicon spp.