Abstract: A transactivation system and method for producing a foreign polypeptide of interest in cells of a host plant is disclosed. The transactivation system has two components. It has genetically transformed cells of the host plant having integrated in their nuclear genome, an inactive or silenced foreign nucleic acid sequence, which is capable of encoding, upon its activation, the foreign polypeptide of interest; and a recombinant RNA viral vector capable of infecting the cells of the host plant and encoding therein a factor for activating or facilitating the expression of inactive or silenced foreign nucleic acid sequence.

Abstract: The present invention relates to the intersection of the fields of immunology and protein engineering, and particularly to antigens and vaccines useful in prevention of infection by Bacillus anthracis. Provided are recombinant protein antigens, compositions, and methods for the production and use of such antigens and vaccine compositions.

Abstract: The present invention provides systems and methods for generating clonal root lines, clonal root cell lines, clonal plant cell lines, and clonal plants and for expressing gene products in such cell lines and plants. In some embodiments, a viral vector containing a polynucleotide of interest operably linked to a promoter is introduced into a plant or portion thereof to generate clonal root lines, clonal root cell lines, clonal plant cell lines, and clonal plants. According to certain inventive methods, a viral vector containing a polynucleotide of interest operably linked to a promoter is introduced into cells of a plant cell line that is maintained in culture to generate clonal plant cell lines and clonal plants. The invention provides clonal root lines, clonal root cell lines, clonal plant cell lines, and clonal plants generated using inventive methods.

Abstract: The present invention relates to the intersection of the fields of immunology and protein engineering, and particularly to antigens and vaccines useful in prevention of infection by Bacillus anthracis. Provided are recombinant protein antigens, compositions, and methods for the production and use of such antigens and vaccine compositions.

Abstract: This invention relates to methods and compositions for obtaining Arabidopsis and Brassica plants. Specifically, the method provides culturing protocols and compositions that facilitate the regeneration of transformed plants following delivery of beneficial DNA molecules.

Abstract: The present invention relates to compositions and systems for expressing pharmaceutically active gene products in plants. In particular, the invention provides compositions, systems and methods for the production of human growth hormone in plants. Provided are nucleic acid and protein sequences, expression and vector constructs, host cells and plants capable of expressing human growth hormone, and compositions and kits comprising produced human growth hormone. Additionally provided are methods for production and use of the compositions of the invention.

Abstract: The present invention provides systems and methods for generating clonal root lines, clonal root cell lines, clonal plant cell lines, and clonal plants and for expressing gene products in such cell lines and plants. According to certain of the inventive methods, a viral vector containing a polynucleotide of interest operably linked to a promoter is introduced into a plant or portion thereof. Plant material (e.g., leaf portions) containing the viral vector are used to generate clonal root lines, e.g., by contacting them with A. rhizogenes so as to form hairy roots. The hairy roots are derived from single ancestral cells and are therefore clonal. These clonal root lines contain the viral vector and express the polynucleotide of interest. Clonal root cell lines, clonal plant cell lines, and clonal plants are obtained from the clonal roots.

Abstract: A transactivation system and method for producing a foreign polypeptide of interest in cells of a host plant is disclosed. The transactivation system has two components. It has genetically transformed cells of the host plant having integrated in their nuclear genome, an inactive or silenced foreign nucleic acid sequence, which is capable of encoding, upon its activation, the foreign polypeptide of interest; and a recombinant RNA viral vector capable of infecting the cells of the host plant and encoding therein a factor for activating or facilitating the expression of inactive or silenced foreign nucleic acid sequence.

Abstract: This invention relates to methods and compositions for obtaining Arabidopsis and Brassica plants. Specifically, the method provides culturing protocols and compositions that facilitate the regeneration of transformed plants following delivery of beneficial DNA molecules.

Abstract: The invention provides methods that make it possible to take advantage of various growth parameters of Arabidopsis in order to grow dense populations of the plant in controlled indoor environments for the purpose of harvesting the biomass and isolating proteins, particularly recombinant proteins suitably for pharmaceutical applications.