Abstract: A computer-implemented method for determining survey sampling parameters for species marker detection comprises receiving a species selection identifying selected species and receiving environmental specifications for an environment to be tested for the species. A sampling plan is generated using the environmental specifications and the species selection, and detectability prediction(s) are generated using the environmental specifications, the species selection, and the current sampling plan to predict whether species marker(s) for the selected species are detectable according to the current sampling plan. Where at least one species marker is undetectable according to the current sampling plan, the process iterates, with each subsequent iteration incorporating an increase in the total volume to be sampled, until either every species marker is detectable according to the then-current sampling plan or an iteration stop limit is reached.

Abstract: A computer-implemented method for determining survey sampling parameters for species marker detection comprises receiving a species selection identifying selected species and receiving environmental specifications for an environment to be tested for the species. A sampling plan is generated using the environmental specifications and the species selection, and detectability prediction(s) are generated using the environmental specifications, the species selection, and the current sampling plan to predict whether species marker(s) for the selected species are detectable according to the current sampling plan. Where at least one species marker is undetectable according to the current sampling plan, the process iterates, with each subsequent iteration incorporating an increase in the total volume to be sampled, until either every species marker is detectable according to the then-current sampling plan or an iteration stop limit is reached.

Abstract: A computer implemented method for determining survey sampling parameters for environmental DNA (eDNA) detection comprises receiving a species selection identifying selected species and receiving environmental specifications for an environment to be tested for presence of the species. A sampling plan is generated using the environmental specifications and the species selection, and detectability prediction(s) are generated using the environmental specifications, the species selection and the current sampling plan to predict whether the selected species is detectable in the environment according to the current sampling plan. Where at least one respective selected species is undetectable according to the current sampling plan, the process iterates, with each subsequent iteration incorporating an increase in the total volume to be sampled, until either every respective selected species is detectable according to the then-current sampling plan or an iteration stop limit is reached.