Abstract: An assay for the determination of the presence or absence of E. histolytica and for differentiation of these from other types of amoebae, as well as for the determination of whether the E. histolytica belongs to a symptomatic or asymptomatic strain, is carried out by means of a DNA-probe adapted to selectively hybridize only to the DNA of the amoeba tested. The probes being used selectively hybridize with, and thus diagnose the presence of, symptomatic (pathogenic) and asymptomatic (non-pathogenic) strains, respectively.

Abstract: A container assembly for facilitating treatment of a viscous test specimen including a cylindrical container body with a cover defining on the interior side thereof a specimen recess for receiving a predetermined volume of specimen material. The specimen recess is defined by a generally cylindrical projecting part which fits into a cylindrical receiving space in the container in a manner whereby excess specimen material adhering to the periphery of the projecting part is automatically wiped from the cover and deposited in an annular space defined between the cover and the container which is sealed when the cover is in a closed position. A second cover may be provided at an opposite end of the container through which a helically formed homogenization rod may be inserted operative during subsequent agitation of the container to dislodge specimen material from the recess. The container may also be inserted directly into a centrifuge.