Abstract: A method of obtaining a plant cell having increased pathogen resistance and/or abiotic stress tolerance is provided, the method comprising the steps of a) providing a plant cell, and b) modifying the genome of the plant cell so as to obtain a decreased or inactivated expression of a protein having an amino acid sequence in which at least one part of the amino acid sequence has at least 78%, such as at least 85%, more preferably at least 90% sequence identity to SEQ ID NO: 5, and wherein the protein comprises less than 200 amino acids. A method of obtaining a plant, as well as a plant cell and a plant, are also provided.

Abstract: A sugar beet extract (SBE) induced defense in potato plants under green-house conditions and in field trial, without apparent toxicity for the plant. Treatment with SBE resulted in significant reduction of the size of Phytophthora infestans infection lesions and lower sporangia production. SBE had no apparent toxic effect, neither on the hyphal growth of the pathogennor on the germination of sporangia. SBE triggered PR-1 protein induction which suggests that the defense mechanism conferred by SBE could be via direct induced resistance. An array of phenolic metabolites and minerals was found in the SBE and constitutes potential inducers of the defense.

Abstract: A sugar beet extract (SBE) induced defense in potato plants under green-house conditions and in field trial, without apparent toxicity for the plant. Treatment with SBE resulted in significant reduction of the size of Phytophthora infestans infection lesions and lower sporangia production. SBE had no apparent toxic effect, neither on the hyphal growth of the pathogennor on the germination of sporangia. SBE triggered PR-1 protein induction which suggests that the defense mechanism conferred by SBE could be via direct induced resistance. An array of phenolic metabolites and minerals was found in the SBE and constitutes potential inducers of the defense.

Abstract: The present invention relates generally to novel genetic sequences that encode fatty acid epoxygenase enzymes, in particular fatty acid ?12-epoxygenase enzymes from plants that are mixed function monooxygenase enzymes. More particularly, the present invention exemplifies cDNA sequences from Crepis spp. and Vernonia galamensis that encode fatty acid ?12-epoxygenases. The genetic sequences of the present invention provide the means by which fatty acid metabolism may be altered or manipulated in organisms, such as, for example, yeasts, moulds, bacteria, insects, birds, mammals and plants, and more particularly in plants. The invention also extends to genetically modified oil-accumulating organisms transformed with the subject genetic sequences and to the oils derived therefrom. The oils thus produced provide the means for the cost-effective raw materials for use in the efficient production of coatings, resins, glues, plastics, surfactants and lubricants.

Abstract: The present invention relates to the isolation, identification and characterization of nucleotide sequences encoding an enzyme catalysing the transfer of fatty acids from phospholipids to diacylglycerol in the biosynthetic pathway for the production of triacylglycerol, to said enzymes and process for the production of triacylglycerols.

Abstract: The present invention relates generally to novel genetic sequences that encode fatty acid epoxygenase enzymes, in particular fatty acid ?12-epoxygenase enzymes from plants that are mixed function monooxygenase enzymes. More particularly, the present invention exemplifies cDNA sequences from Crepis spp. and Vernonia galamensis that encode fatty acid ?12-epoxygenases. The genetic sequences of the present invention provide the means by which fatty acid metabolism may be altered or manipulated in organisms, such as, for example, yeasts, moulds, bacteria, insects, birds, mammals and plants, and more particularly in plants. The invention also extends to genetically modified oil-accumulating organisms transformed with the subject genetic sequences and to the oils derived therefrom. The oils thus produced provide the means for the cost-effective raw materials for use in the efficient production of coatings, resins, glues, plastics, surfactants and lubricants.

Abstract: The invention relates to methods for increasing the oil content in plants, preferably in plant seeds, by expressing the Ypr140w polypeptide from yeast or corresponding polypeptides from plants. The invention furthermore relates to expression constructs for expressing the yeast polypeptide Ypr140w or corresponding polypeptides from plants in plants, preferably in plant seeds, the transgenic plants expressing the polypeptide and to the use of the transgenic plants for the production of food, feed, seed, pharmaceuticals or fine chemicals, in particular for the production of oils.

Abstract: The invention relates to methods for increasing the oil content in plants, preferably in plant seeds, by expressing a polypeptide from yeast. The invention furthermore relates to expression constructs for expressing the yeast polypeptide in plants, preferably in plant seeds, the transgenic plants expressing the yeast polypeptide and to the use of said transgenic plants for the production of food, feeds, seed, pharmaceuticals or fine chemicals, in particular for the production of oils.

Abstract: The present invention relates to the production of triacylglycerol in a transformed organism or host cell, by means of a nucleotide sequence from S. cerevisiae, whereby said enzyme catalyzes the transfer of fatty acids from phospholipids to diacylglycerol in any acyl-CoA-independent reaction. The transformation of the organism or host cell results in increased oil content.

Abstract: The present invention relates to the isolation, identification and characterization of nucleotide sequences encoding an enzyme catalysing the transfer of fatty acids from phospholipids to diacylglycerol in the biosynthetic pathway for the production of triacylglycerol, to the said enzymes and a process for the production of triacylglycerols.

Abstract: The present invention relates generally to novel genetic sequences that encode fatty acid epoxygenase enzymes, in particular fatty acid ?12-epoxygenase enzymes from plants that are mixed function monooxygenase enzymes. More particularly, the present invention exemplifies cDNA sequences from Crepis spp. and Vernonia galamensis that encode fatty acid ?12-epoxygenases. The genetic sequences of the present invention provide the means by which fatty acid metabolism may be altered or manipulated in organisms, such as, for example, yeasts, moulds, bacteria, insects, birds, mammals and plants, and more particularly in plants. The invention also extends to genetically modified oil-accumulating organisms transformed with the subject genetic sequences and to the oils derived therefrom. The oils thus produced provide the means for the cost-effective raw materials for use in the efficient production of coatings, resins, glues, plastics, surfactants and lubricants.

Abstract: The invention relates to methods for increasing the oil content in plants, preferably in plant seeds, by expressing the Ypr140w polypeptide from yeast or corresponding polypeptides from plants. The invention furthermore relates to expression constructs for expressing the yeast polypeptide Ypr140w or corresponding polypeptides from plants in plants, preferably in plant seeds, the transgenic plants expressing the polypeptide and to the use of said transgenic plants for the production of food, feed, seed, pharmaceuticals or fine chemicals, in particular for the production of oils.

Abstract: The invention relates to methods for increasing the oil content in plants, preferably in plant seeds, by expressing a polypeptide from yeast. The invention furthermore relates to expression constructs for expressing the yeast polypeptide in plants, preferably in plant seeds, the transgenic plants expressing the yeast polypeptide and to the use of said transgenic plants for the production of food, feeds, seed, pharmaceuticals or fine chemicals, in particular for the production of oils.

Abstract: The present invention relates to the use of an enzyme mixture containing at least one enzyme with phospholipid:diacylglycerol acyltransferase activity for the production of plant storage lipids containing polyunsaturated fatty acids.

Abstract: The present invention relates to the use of a novel enzyme and its encoding gene for transformation. More specifically, the invention relates to the use of a gene encoding an enzyme with acyl-CoA: diacylglycerol acyltransferase activity. This gene expressed alone in transgenic organisms will increase the total amount of oil (i.e. triacylglycerols) that is produced.

Abstract: The present invention relates to the use of a novel enzyme and its encoding gene for transformation. More specifically, the invention relates to the use of a gene encoding an enzyme with acyl-CoA:diacylglycerol acyltransferase activity. This gene expressed alone in transgenic organisms will increase the total amount of oil (i.e. triacylglycerols) that is produced.

Abstract: The present invention relates generally to novel genetic sequences that encode fatty acid epoxygenase enzymes, in particular fatty acid &Dgr;12-epoxygenase enzymes from plants that are mixed function monooxygenase enzymes. More particularly, the present invention exemplifies cDNA sequences from Crepis spp. and Vernonia galamensis that encode fatty acid &Dgr;12-epoxygenases. The genetic sequences of the present invention provide the means by which fatty acid metabolism may be altered or manipulated in organisms, such as, for example, yeasts, moulds, bacteria, insects, birds, mammals and plants, and more particularly in plants. The invention also extends to genetically modified oil-accumulating organisms transformed with the subject genetic sequences and to the oils derived therefrom. The oils thus produced provide the means for the cost-effective raw materials for use in the efficient production of coatings, resins, glues, plastics, surfactants and lubricants.

Abstract: The present invention relates to a novel plant enzyme called delta 12 fatty acid acetylenase. This enzyme is responsible for the conversion of fatty acids to acetylenic acids and the invention relates to production of such acids. The invention also relates to use of cDNA encoding acetylenase, preferably Crepis alpina delta 12 acetylenase, for transforming organisms such as oil accumulating organisms selected from the group consisting of oil crops, oleogeneous yeasts and moulds. Furthermore, the invention relates to organisms such as oil accumulating organisms transformed with acetylenase cDNA, and to oils and other acetylenic compounds from said organisms.

Abstract: The present invention relates generally to novel genetic sequences which encode fatty acid epoxygenase enzymes. In particular, the present invention relates to genetic sequences which encode fatty acid &Dgr;12-epoxygenase enzymes comprising mixed function monooxygenase enzymes. More preferably, the present invention provides cDNA sequences which encode plant fatty acid epoxygenases, in particular the Crepis palaestina &Dgr;12-epoxygenase and homologues, analogues and derivatives thereof. The genetic sequences of the present invention provide the means by which fatty acid metabolism may be altered or manipulated in organisms such as yeasts, moulds, bacteria, insects, birds, mammals and plants, in particular to convert unsaturated fatty acids to epoxygenated fatty acids therein. The invention extends to genetically modified oil-accumulating organisms transformed with the subject genetic sequences and to the oils derived therefrom.