Abstract: A multi-layer film comprising: (i) a polyester base layer (B) having a first and second surface wherein the polyester of the base layer is a crystals isabie polyester; and (ii) a heat-sealabie copolyester layer (A) disposed on one or both surfaces of said polyester base layer (B); wherein the polyester base layer (S) comprises titanium dioxide particles in an amount of from about 1 to about 30 wt % by total weight of the base layer, wherein said particles are coated with an organic coating; and a multi-layer card comprising said multi-layer film, a polymeric inlay layer and a polymeric overlay layer such that the layer order is polymeric inlay layer, first multi-layer film and first polymeric overlay layer.

Abstract: A heat-sealable polymeric film includes a substrate layer of polymeric material having on a surface thereof a heat-sealable coating layer. The layer includes a copolyester having a degree of crystallinity of at least 5%, and is less than 8 ?m in thickness. The copolyester consists essentially of repeat units of one or more aromatic dicarboxylic acids, one or more aliphatic dicarboxylic acids, and a stoichiometric amount of a glycol component consisting of one or more glycols selected from the group consisting of aliphatic glycols and cycloaliphatic glycols.

Abstract: Bovine respiratory disease (BRD) complex, shipping fever, or pneumomic pasteurellosis, is a multifactorial disease whereby a combination of viral infection, adverse environment and poor immune status may combine to predispose animals to bacterial infections. The exotoxin, or leukotoxin (Lkt), may contribute to pathogenesis by impairing the primary lung defenses and subsequent immune responses or by causing inflammations as a result of leukocyte lysis. The present invention provides a modified microorganism which produces an Lkt toxin, wherein said Lkt toxin is partially or fully inactivated. In a further embodiment of the present invention, there is provided a modified microorganism wherein an Lkt toxin operon including an Lkt structural gene and/or a post transational activator of the organism is partially or fully inactivated. The present applicants have found that a precursor of Lkt toxin has reduced toxic activity.

Abstract: Disclosed herein is a modified microorganism which produces an RTX toxin, wherein said RTX toxin is partially or fully inactivated. Also disclosed is a modified microorganism wherein an RTX toxin gene including an RTX structural gene and/or a post-translational activator of the organism is partially or full inactivated. As disclosed, the precursor of an RTX toxin has reduced toxic activity.

Abstract: The present invention provides a modified microorganism which produces an RTX toxin, wherein said RTX toxin is partially or fully inactivated. In a further embodiment of the present invention, there is provided a modified microorganism wherein an RTX toxin gene including an RTX structural gene and/or a post-translational activator of the organism is partially or fully inactivated. The present applicants have found that a precursor of an RTX toxin has reduced toxic activity. Surprisingly, the RTX toxin precursor is capable of inducing an immune response in an animal that offers cross protection against heterologous challenge with a microorganism which produces the RTX toxin.