Patents by Inventor Mark A. Mellon
Mark A. Mellon has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230028975Abstract: The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction.Type: ApplicationFiled: October 13, 2021Publication date: January 26, 2023Inventors: Elena Brevnova, John E. McBride, Erin Wiswall, Kevin S. Wenger, Nicky Caiazza, Heidi Hau, Aaron Argyros, Frank Agbogbo, Charles F. Rice, Trisha Barrett, John S. Bardsley, Abigail Foster, Anne K. Warner, Mark Mellon, Ryan Skinner, Indraneel Shikhare, Riaan Den Haan, Chhayal V. Gandhi, Alan Belcher, Vineet B. Rajgarhia, Allan C. Froehlich, Kristen M. Deleault, Emily Stonehouse, Shital A. Tripathi, Jennifer Gosselin, Yin-Ying Chiu, Haowen Xu
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Publication number: 20210388398Abstract: The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterologous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability of Kluyveromyces to produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.Type: ApplicationFiled: December 22, 2020Publication date: December 16, 2021Inventors: John McBride, Elena Brevnova, Mark Mellon, Allan Froehlich, Kristen Deleault, Vineet Rajgarhia, Riaan Den Haan, Merja Penttila, Marja Ilmen, Matti Siika-Aho, Jaana Uusitalo, Emily A. Stonehouse, Alan Gilbert, Haowen Xu, Deidre Willies, John Bardsley, Anu Koivula, Sanni Voutilainen
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Patent number: 11193274Abstract: A protective barrier device adapted for placement within a structure with a particular use adjacent to a vehicle. The device includes a pair of base members comprised of a hollow housing having a cavity for the addition of a weighted material. The device including a top rail and a bottom rail assembled from sections and coupled to the base members to define a perimeter of the device. A central portion generally comprising a panel is shaped for receipt within the perimeter and coupled to the rails in a cohesive assembly. The device is configured for assembly from a collapsed to an extended position for easily transport and shipping.Type: GrantFiled: September 25, 2018Date of Patent: December 7, 2021Inventor: Mark Mellon
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Patent number: 11193130Abstract: The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction.Type: GrantFiled: May 30, 2019Date of Patent: December 7, 2021Assignees: Lallemand Hungary Liquidity Management LLC, Stellenbosch UniversityInventors: Elena Brevnova, John E. McBride, Erin Wiswall, Kevin S. Wenger, Nicky Caiazza, Heidi Hau, Aaron Argyros, Frank Agbogbo, Charles F. Rice, Trisha Barrett, John S. Bardsley, Abigail Foster, Anne K. Warner, Mark Mellon, Ryan Skinner, Indraneel Shikhare, Riaan Den Haan, Chhayal V. Gandhi, Alan Belcher, Vineet B. Rajgarhia, Allan C. Froehlich, Kristen M. Deleault, Emily Stonehouse, Shital A. Tripathi, Jennifer Gosselin, Yin-Ying Chiu, Haowen Xu
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Publication number: 20200095592Abstract: The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction.Type: ApplicationFiled: May 30, 2019Publication date: March 26, 2020Inventors: Elena Brevnova, John E. McBride, Erin Wiswall, Kevin S. Wenger, Nicky Caiazza, Heidi Hau, Aaron Argyros, Frank Agbogbo, Charles F. Rice, Trisha Barrett, John S. Bardsley, Abigail Foster, Anne K. Warner, Mark Mellon, Ryan Skinner, Indraneel Shikhare, Riaan Den Haan, Chhayal V. Gandhi, Alan Belcher, Vineet B. Rajgarhia, Allan C. Froehlich, Kristen M. Deleault, Emily Stonehouse, Shital A. Tripathi, Jennifer Gosselin, Yin-Ying Chiu, Haowen Xu
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Patent number: 10428322Abstract: The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.Type: GrantFiled: January 10, 2019Date of Patent: October 1, 2019Assignee: Lallemand Hungary Liquidity Management LLCInventors: Elena E. Brevnova, Vineet Rajgarhia, Mark Mellon, Anne Warner, John McBride, Chhayal Gandhi, Erin Wiswall
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Patent number: 10385345Abstract: The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction.Type: GrantFiled: May 2, 2017Date of Patent: August 20, 2019Assignees: Lallemand Hungary Liquidity Management LLC, Universiteit StellenboschInventors: Elena Brevnova, John E. McBride, Erin Wiswall, Kevin S. Wenger, Nicky Caiazza, Heidi Hau, Aaron Argyros, Frank Agbogbo, Charles F. Rice, Trisha Barrett, John S. Bardsley, Abigail Foster, Anne K. Warner, Mark Mellon, Ryan Skinner, Indraneel Shikhare, Riaan Den Haan, Chhayal V. Gandhi, Alan Belcher, Vineet B. Rajgarhia, Allan C. Froehlich, Kristen M. Deleault, Emily Stonehouse, Shital A. Tripathi, Jennifer Gosselin, Yin-Ying Chiu, Haowen Xu
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Patent number: 10294484Abstract: The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction.Type: GrantFiled: November 10, 2015Date of Patent: May 21, 2019Assignees: Lallemand Hungary Liquidity Management LLC, Stellenbosch UniversityInventors: Elena Brevnova, John E. McBride, Erin Wiswall, Kevin S. Wenger, Nicky Caiazza, Heidi Lau, Aaron Argyros, Frank Agbogbo, Charles F. Rice, Trisha Barrett, John S. Bardsley, Abigail Foster, Anne K. Warner, Mark Mellon, Ryan Skinner, Indraneel Shikhare, Riaan Den Haan, Chhayal V. Gandhi, Alan Belcher, Vineet B. Rajgarhia, Allan C. Froehlich, Kristen M. Deleault, Emily Stonehouse, Shital A. Tripathi, Jennifer Gosselin, Yin-Ying Chiu, Haowen Xu
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Publication number: 20190127714Abstract: The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.Type: ApplicationFiled: January 10, 2019Publication date: May 2, 2019Inventors: Elena E. Brevnova, Vineet Rajgarhia, Mark Mellon, Anne Warner, John McBride, Chhayal Gandhi, Erin Wiswall
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Publication number: 20190093346Abstract: A protective barrier device adapted for placement within a structure with a particular use adjacent to a vehicle. The device includes a pair of base members comprised of a hollow housing having a cavity for the addition of a weighted material. The device including a top rail and a bottom rail assembled from sections and coupled to the base members to define a perimeter of the device. A central portion generally comprising a panel is shaped for receipt within the perimeter and coupled to the rails in a cohesive assembly. The device is configured for assembly from a collapsed to an extended position for easily transport and shipping.Type: ApplicationFiled: September 25, 2018Publication date: March 28, 2019Inventor: Mark Mellon
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Patent number: 10214733Abstract: The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.Type: GrantFiled: November 28, 2017Date of Patent: February 26, 2019Assignee: Lallemand Hungary Liquidity Management LLCInventors: Elena E. Brevnova, Vineet Rajgarhia, Mark Mellon, Anne Warner, John McBride, Chhayal Gandhi, Erin Wiswall
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Publication number: 20180258449Abstract: The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterologous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability of Kluyveromyces to produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.Type: ApplicationFiled: May 21, 2018Publication date: September 13, 2018Inventors: John McBride, Elena Brevnova, Mark Mellon, Allan Froehlich, Kristen Deleault, Vineet Rajgarhia, Riaan Den Haan, Merja Penttila, Marja Ilmen, Matti Siika-Aho, Jaana Uusitalo, Emily A. Stonehouse, Alan Gilbert, Haowen Xu, Deidre Willies, John Bardsley, Anu Koivula, Sanni Voutilainen
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Publication number: 20180155700Abstract: The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.Type: ApplicationFiled: November 28, 2017Publication date: June 7, 2018Inventors: Elena E. Brevnova, Vineet Rajgarhia, Mark Mellon, Anne Warner, John McBride, Chhayal Gandhi, Erin Wiswall
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Patent number: 9988652Abstract: The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterologous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability of Kluveryomyces to produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.Type: GrantFiled: July 1, 2015Date of Patent: June 5, 2018Assignees: Lallemand Hungary Liquidity Management LLC, Stellenbosch UniversityInventors: John McBride, Elena Brevnova, Mark Mellon, Allan Froehlich, Kristen Deleault, Vineet Rajgarhia, Riaan Den Haan, Merja Penttila, Marja Ilmen, Matti Siika-Aho, Jaana Uusitalo, Emily A. Stonehouse, Alan Gilbert, Haowen Xu, Deidre Willes, John Bardsley, Anu Koivula, Sanni Voutilainen
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Patent number: 9856465Abstract: The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.Type: GrantFiled: March 8, 2016Date of Patent: January 2, 2018Assignee: Lallemand Hungary Liquidity Management LLCInventors: Elena E. Brevnova, Vineet Rajgarhia, Mark Mellon, Anne Warner, John McBride, Chhayal Gandhi, Erin Wiswall
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Publication number: 20170240906Abstract: The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction.Type: ApplicationFiled: May 2, 2017Publication date: August 24, 2017Inventors: Elena Brevnova, John E. McBride, Erin Wiswall, Kevin S. Wenger, Nicky Caiazza, Heidi Hau, Aaron Argyros, Frank Agbogbo, Charles F. Rice, Trisha Barrett, John S. Bardsley, Abigail Foster, Anne K. Warner, Mark Mellon, Ryan Skinner, Indraneel Shikhare, Riaan Den Haan, Chhayal V. Gandhi, Alan Belcher, Vineet B. Rajgarhia, Allan C. Froehlich, Kristen M. Deleault, Emily Stonehouse, Shital A. Tripathi, Jennifer Gosselin, Yin-Ying Chiu, Haowen Xu
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Publication number: 20160177283Abstract: The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.Type: ApplicationFiled: March 8, 2016Publication date: June 23, 2016Inventors: Elena E. BREVNOVA, Vineet RAJGARHIA, Mark MELLON, Anne WARNER, John MCBRIDE, Chhayal GANDHI, Erin WISWALL
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Patent number: 9315833Abstract: The present invention relates to the engineering and expression of heterologous cellulosomes in microorganisms in order to facilitate the conversion of biomass to useful products. In some embodiments, the invention relates to the expression of scaffoldin proteins which form the nucleus of a cellulosome. Cellulases or other biomass-degrading enzymes can be non-covalently linked to the scaffoldin protein by virtue of a dockerin domain-cohesin domain interaction.Type: GrantFiled: February 18, 2010Date of Patent: April 19, 2016Assignees: Lallemand Hungary Liquidity Management LLC, Stellenbosch UniversityInventors: John McBride, Mark Mellon, Vineet Rajgarhia, Elena E. Brevnova, Erin Wiswall, David A. Hogsett, Danie LaGrange, Shaunita Rose, Emile Van Zyl
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Publication number: 20160068850Abstract: The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction.Type: ApplicationFiled: November 10, 2015Publication date: March 10, 2016Inventors: Elena Brevnova, John E. McBride, Erin Wiswall, Kevin S. Wenger, Nicky Caiazza, Heidi Lau, Aaron Argyros, Frank Agbogbo, Charles F. Rice, Trisha Barrett, John S. Bardsley, Abigail Foster, Anne K. Warner, Mark Mellon, Ryan Skinner, Indraneel Shikhare, Riaan Den Haan, Chhayal V. Gandhi, Alan Belcher, Vineet B. Rajgarhia, Allan C. Froehlich, Kristen M. Deleault, Emily Stonehouse, Shital A. Tripathi, Jennifer Gosselin, Yin-Ying Chiu, Haowen Xu
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Publication number: 20160010117Abstract: The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability of Kluveryomyces to produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.Type: ApplicationFiled: July 1, 2015Publication date: January 14, 2016Inventors: John McBride, Elena Brevnova, Mark Mellon, Allan Froehlich, Kristen Deleault, Vineet Rajgarhia, Riaan Den Haan, Merja Penttila, Marja Ilmen, Matti Siika-Aho, Jaana Uusitalo, Emily A. Stonehouse, Alan Gilbert, Haowen Xu, Deidre Willes, John Bardsley, Anu Koivula, Sanni Voutilainen