Abstract: The present invention relates to a composition comprising a 1,3-selective enzymatic intraesterified oil product having a greater unsaturated-saturated-unsaturated (USU) fatty acid content on the glycerol moiety than the oil from which it was derived. In an embodiment, the starting oil is cottonseed oil and in USU the U is primarily linoleic acid and the S is primarily palmitic acid. Further, the present invention relates to a method for increasing the USU content in an oil, comprising performing 1,3-selective enzymatic intraesterification of a regular starting oil wherein saturated-unsaturated-saturated (SUS) content is greater than the USU content in the starting oil prior to performing 1,3-selective enzymatic intraesterification. Due to the greater USU content, the 1,3-selective enzymatic intraesterified oil product has an increased melting temperature and solid fat content and therefore useful for margarines and food spreads as compared to the oil it was produced from.

Abstract: A process for separation of components of vegetable oils is disclosed. The process utilizes a silica column of a particular size that allows for sufficient sample size and separation to provide good reproducibility, and also provides for quantitation of the polar and non-polar components of the oils. Samples are eluted from the silica column using a petroleum ether:diethyl ether solution of approximately 87:13.