Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

Abstract: A method and system for analysis of a digital core image obtained from a sample are disclosed. The method includes performing segmentations on the digital core image using multiple approaches to obtain multiple segmented images which are statistically analyzed to select the most suitable approach of the multiple approaches. Thereafter, a digital core model is generated using the segmented image corresponding to the most suitable approach. A simulation test may be performed on the digital core model to obtain a model test result and an oilfield operation may be performed based on the model test result. The system includes measurement and testing equipment to obtain the digital core image and a computing system including a data repository for storing a digital core image and a digital core model, and a digital core modeling tool. The digital core modeling tool performs the segmentations, statistical analysis, and generates the digital core model.

Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

Abstract: The present invention provides methods, reagents and kits for carrying out a variety of assays suitable for analyzing polynucleotides or samples that include an amplification step performed in a multiplex fashion. Also provided are methods for analyzing and improving the efficiency of amplification and for carrying out gene expression analysis.

Abstract: Methods, compositions and kits are described for resequencing, confirming or verifying Next Generation Sequencing (NGS) results with Sanger Sequencing. These methods are particularly useful for samples having very limited quantities such as formalin-fixed, paraffin-embedded (FFPE), Laser Capture Microdissection (LCM), fine needle biopsies or aspirates.

Abstract: An alignment apparatus is coupled to an accessory module that is rotatably attachable to an electronic device. The alignment apparatus includes a pivot mounting member and a connector retaining member movably coupled to the pivot mounting member. The connector retaining member is rigidly coupled to a connector. The connector retaining member moves the connector with respect to the pivot mounting member to a predetermined angle. The predetermined angle aligns the connector with a connecting mate on the electronic device in an approximately parallel position while the accessory module is rotating for attachment onto the electronic device.

Abstract: A method and system for analysis of a digital core image obtained from a sample are disclosed. The method includes performing segmentations on the digital core image using multiple approaches to obtain multiple segmented images which are statistically analyzed to select the most suitable approach of the multiple approaches. Thereafter, a digital core model is generated using the segmented image corresponding to the most suitable approach. A simulation test may be performed on the digital core model to obtain a model test result and an oilfield operation may be performed based on the model test result. The system includes measurement and testing equipment to obtain the digital core image and a computing system including a data repository for storing a digital core image and a digital core model, and a digital core modeling tool. The digital core modeling tool performs the segmentations, statistical analysis, and generates the digital core model.

Abstract: A method for performing a simulation of a field having a subterranean formation is described. The method includes obtaining phase behavior data of subterranean fluids of the field, generating an equation of state (EOS) model of the fluids based on the phase behavior data, generating a Helmholtz free energy model that reproduces predictions of the EOS model over a pre-determined pressure and temperature range, and performing the simulation of the field using the Helmholtz free energy model. The method may further include reducing the EOS model to a reduced EOS model having a reduced number of components to represent the EOS model over a pre-determined pressure and temperature range, generating the Helmholtz free energy model based on the reduced EOS model, and obtaining and using phase behavior data of injection fluids used. A computer system data.

Abstract: A system and method for showing heterogeneity of a porous sample by evaluating the porous sample to generate a digital core image, performing segmentations on the digital core image using multiple approaches to obtain a segmented volume, dividing the segmented volume into one or more sub-volumes of differing size, calculating one or more petrophysical or fluid flow parameters or porosity from the one or more sub-volumes, and presenting data as a structure composed of grid blocks representing an exact representation of the one or more sub-volumes positioned according to a spatial location of the one or more sub-volumes based on selected parameters or values from the one or more petrophysical or fluid flow parameters or porosity.

Abstract: A method and computer system for performing simulation of a field having a subterranean formation. The method includes obtaining measured core sample data of a core sample retrieved from the formation, the core sample data measured by injecting fluid into the core sample, obtaining a digital rock model of the core sample describing a physical pore structure in the core sample, and obtaining a fluid model describing a physical property of the fluid. A digital core analysis (DCA) of the core sample is performed to generate a DCA simulation result and the DCA is tuned using the measured core sample data to reduce a difference between the DCA simulation result and the measured core sample data. The tuning is performed by adjusting, in response to the difference exceeding a pre-determined limit, a parameter of the DCA to generate an adjusted parameter and further performing the DCA to reduce the difference.

Abstract: Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample.

Abstract: A method for performing simulation of a field having a subterranean formation, including: obtaining a three-dimensional (3D) porous solid image of a core sample, the core sample representing a portion of the field; generating a digital rock model from the solid image, the digital rock model describing a physical pore structure in the core sample; obtaining phase behavior data of fluids of the field; generating a digital fluid model of the fluids based on the phase behavior data, the digital fluid model describing a physical property of the fluid; performing, on a computer system and based on the digital rock model and the digital fluid model, simulations of the field by varying an input parameter for the simulations; and analyzing an output parameter generated by the simulations to determine an effect of varying the input parameter on the output parameter.

Abstract: In some embodiments, the disclosure relates generally to methods, compositions, systems, apparatuses and kits comprising a multiplex nucleic acid amplification reaction that employs a plurality (e.g., hundreds, thousands, tens-of-thousands or hundreds-of-thousands) of different target-specific primer pairs that enable substantially simultaneous amplification of a plurality of different target sequences-of-interest in a single reaction mixture. In some embodiments, the multiplex nucleic acid amplification reaction generates a plurality of amplicons having sequences derived from a sample containing RNA or DNA, including whole transcriptome or genomic samples. In some embodiments, the sequences and abundances of at least some of the plurality of amplicons are characterized, optionally simultaneously or through a single assay, by suitable detection methods, including sequencing or other procedures known in the art.

Abstract: An alignment apparatus is coupled to an accessory module that is rotatably attachable to an electronic device. The alignment apparatus includes a pivot mounting member and a connector retaining member movably coupled to the pivot mounting member. The connector retaining member is rigidly coupled to a connector. The connector retaining member moves the connector with respect to the pivot mounting member to a predetermined angle. The predetermined angle aligns the connector with a connecting mate on the electronic device in an approximately parallel position while the accessory module is rotating for attachment onto the electronic device.

Abstract: Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample.

Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for determining copy number variation of one or more nucleic acids present in a sample. In some aspects, the method includes various target-specific primers that allow for the selective amplification of one or more target nucleic acids in the sample. In yet another aspect, the invention relates to determining copy number variation with respect to gene or chromosome representation of a nucleic acid in the sample. In some aspects, the method for determining copy number variation of different target nucleic acids in a sample using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including diagnosis, predictive therapeutic regimes or other therapeutic purposes.

Abstract: Methods of well treatment include modeling approaches that account for the effect of pore structure during well stimulation treatments. In one aspect, methods may include preparing a computer model of a porous medium; simulating an injection of a fluid into the computer model of the porous medium; measuring a pore-scale heterogeneity of the computer model of the porous medium, and designing a stimulating fluid treatment for the porous medium. Other aspects may include the development of a wellbore stimulation methodology that allows stimulation fluid breakthrough curves of differing formation samples to be plotted as a single curve that accounts for the varied pore structure of the respective samples.

Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the concentration of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture. In some aspects, nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing.

Abstract: In some embodiments, the disclosure relates generally to methods, compositions, systems, apparatuses and kits comprising a multiplex nucleic acid amplification reaction that employs a plurality (e.g., hundreds, thousands, tens-of-thousands or hundreds-of-thousands) of different target-specific primer pairs that enable substantially simultaneous amplification of a plurality of different target sequences-of-interest in a single reaction mixture. In some embodiments, the multiplex nucleic acid amplification reaction generates a plurality of amplicons having sequences derived from a sample containing RNA or DNA, including whole transcriptome or genomic samples. In some embodiments, the sequences and abundances of at least some of the plurality of amplicons are characterized, optionally simultaneously or through a single assay, by suitable detection methods, including sequencing or other procedures known in the art.