Abstract: This invention provides the field of therapeutics. Most specifically present invention provides methods of generating in vitro engineered dendritic cells conditionally expressing interleukin-12 (IL-12) under the control of a gene expression modulation system in the presence of activating ligand and uses for therapeutic purposes in animals including human.

Abstract: We disclose quantitative geometrical analysis enabling the measurement of several features of images of tissues including number, size, density of extracted hepatocyte nuclei, and other metrics. Automation of feature extraction creates a high throughput capability that enables analysis of histologically prepared tissue sections for accurate quantification of extracted features from tissues. Measurement results are input into a relational database where they can be statistically analyzed and compared across studies. As part of the integrated process, results are also imprinted on the images themselves to facilitate auditing of the results. The analysis is objective, fast, repeatable and accurate and provides an alternative or supplement to the subjective analysis of tissue slides by a pathologist.