Abstract: An optical imaging system for cell culture monitoring is provided. The system includes an illumination segment having an illumination source and a collimating lens positioned between a first surface of a cell culture vessel and the illumination source. The illumination source and the collimating lens are arranged to transmit light through the first surface at an angle oblique to the first surface of the cell culture vessel. The system also includes a detection segment having a detector and a lens positioned between the first surface of the cell culture vessel and the detector. The lens focuses light to the detector through an aperture stop, and the detector receives light that exits the first surface of the cell culture vessel at an angle oblique to the first surface.

Abstract: A cell monitoring plate comprises a flat surface on which multiple cell culturing vessels may be stacked. The flats surface has multiple optical imaging systems embedded therein to fully image a cell culture vessels stacked on the plate. Each one of the multiple optical imaging systems provides both illumination and imaging through a single aperture in the surface of the monitoring plate.

Abstract: A magnification adjustable projection system includes an imaging system having a first pair of cylindrical lens plates located within an object or image space. The first pair of cylindrical lens plates includes a first cylindrical lens plate axially movable relative to a second cylindrical lens plate. A second pair of cylindrical lens plates is located within the object or image space in optical alignment with the first pair of cylindrical lens plates. The second pair of cylindrical lens plates includes a third cylindrical lens plate axially movable relative to a fourth cylindrical lens plates. First and second actuators adjusts distances between the first and second cylindrical lens plates and between the third and fourth cylindrical lens plates. The first and second pairs of cylindrical lens plates have first and second cylindrical transverse axes that are approximately 45° relative to each other.

Abstract: A cell culture vessel, cell culture monitoring system, and Raman spectroscopy system for non-invasive measuring of a cell culture are provided. The vessel includes a cell culture chamber that operates as a closed system, a wall defining a boundary of the cell culture chamber and separating the interior space of the cell culture chamber from an exterior of the cell culture chamber, and a window disposed in the wall and separating the interior space of the cell culture chamber from an exterior of the cell culture chamber. The cell culture chamber includes an interior space for housing at least one of the cell culture and a cell culture media. The window includes a polymer and allows monitoring of the cell culture via a monitoring module disposed on the exterior without the monitoring module coming into physical contact with the cell culture or the cell culture media.

Abstract: A cell monitoring plate comprises a flat surface on which multiple cell culturing vessels may be stacked. The flats surface has multiple optical imaging systems embedded therein to fully image a cell culture vessels stacked on the plate. Each one of the multiple optical imaging systems provides both illumination and imaging through a single aperture in the surface of the monitoring plate.

Abstract: A method for processing a transparent workpiece includes directing a laser beam oriented along a beam pathway through an aspheric optical element and the transparent workpiece. The laser beam impinges the aspheric optical element radially offset from a centerline axis of the aspheric optical element by an offset distance of 30% the 1/e2 diameter of the laser beam or greater. The beam pathway and the transparent workpiece are tilted relative to one another such that the beam pathway has a beam pathway angle of less than 90° relative to an impingement surface at the impingement surface and a portion of the laser beam directed into the transparent workpiece is a laser beam focal line having an internal focal line angle of less than 80° relative to the impingement surface, such that a defect with a defect angle of less than 80° is formed by induced absorption within the transparent workpiece.

Abstract: A Raman spectroscopy system is provided. The spectroscopy system includes an optical switch including a first side having a pump inlet and a return outlet, and a second side having a plurality of pump outlets and a plurality of return inlets. The spectroscopy system includes at least one radiation source optically coupled to the pump inlet and a detector optically coupled to the return outlet. The spectroscopy system further includes a pump filter module optically coupled between the at least one radiation source and the pump outlets and a return filter module optically coupled between the detector and the return inlets. The spectroscopy system further includes a plurality of probes, each probe optically connected to at least one of the plurality of pump outlets by at least one excitation fiber and optically coupled to one of the return inlets by at least one emission fiber.

Abstract: A Raman spectroscopy system is provided. The spectroscopy system includes an optical switch including a first side having a pump inlet and a return outlet, and a second side having a plurality of pump outlets and a plurality of return inlets. The spectroscopy system includes at least one radiation source optically coupled to the pump inlet and a detector optically coupled to the return outlet. The spectroscopy system further includes a pump filter module optically coupled between the at least one radiation source and the pump outlets and a return filter module optically coupled between the detector and the return inlets. The spectroscopy system further includes a plurality of probes, each probe optically connected to at least one of the plurality of pump outlets by at least one excitation fiber and optically coupled to one of the return inlets by at least one emission fiber.

Abstract: A cell monitoring plate comprises a flat surface on which multiple cell culturing vessels may be stacked. The flats surface has multiple optical imaging systems embedded therein to fully image a cell culture vessels stacked on the plate. Each one of the multiple optical imaging systems provides both illumination and imaging through a single aperture in the surface of the monitoring plate.

Abstract: An optical imaging system for cell culture monitoring is provided. The system includes an illumination segment having an illumination source and a collimating lens positioned between a first surface of a cell culture vessel and the illumination source. The illumination source and the collimating lens are arranged to transmit light through the first surface at an angle oblique to the first surface of the cell culture vessel. The system also includes a detection segment having a detector and a lens positioned between the first surface of the cell culture vessel and the detector. The lens focuses light to the detector through an aperture stop, and the detector receives light that exits the first surface of the cell culture vessel at an angle oblique to the first surface.

Abstract: Optical systems including lens assemblies and methods of imaging fields of view using such optical systems are disclosed. An optical system for imaging a two dimensional field includes a first lens assembly, a first scanning mirror, a second lens assembly, and a two dimensional image sensor. The first lens assembly has a first transform function whose output is within 0.1% of f1*(c1*?1+(1?c1)*sin(?1)) for any ray of light that traverses the first lens assembly from a center of an entrance pupil of the first lens assembly at an angle ?1 relative to an optical axis of the first lens assembly. f1 is a focal length of the first lens assembly, and ?0.5<c1<2.

Abstract: Optical systems including lens assemblies and methods of imaging fields of view using such optical systems are disclosed. An optical system for imaging a two dimensional field includes a first lens assembly, a first scanning mirror, a second lens assembly, and a two dimensional image sensor. The first lens assembly has a first transform function whose output is within 0.1% of f1*(c1*?1+(1?c1)*sin(?1)) for any ray of light that traverses the first lens assembly from a center of an entrance pupil of the first lens assembly at an angle ?1 relative to an optical axis of the first lens assembly. f1 is a focal length of the first lens assembly, and ?0.5<c1<2.