Abstract: A floor mat clip assembly adapted to attach a floor mat to the interior carpet of a vehicle is disclosed. The clip assembly includes a floor clip attached to the carpet and a mat clip attached to the floor mat. The floor clip includes a base member and a top member having a locking member rotatably attached thereto. The base and top member of the floor clip are adapted to cut through the carpet and interlock to sandwich the carpet therebetween. The mat clip includes a top member and a base member, each having an aperture and adapted to interlock to sandwich the mat therebetween. The mat can then be positioned to insert the locking member of the floor clip through the aperture of the mat clip. A subsequent rotation of the locking member locks together the respective clips to secure the floor mat to the interior carpet.

Abstract: Provided is a method of activating an immune cell of a subject with Human Immunodeficiency Virus (HIV), comprising contacting the immune cell with a phorbol ester and a calcium ionophore. Also provided is a composition comprising immune cells of a subject diagnosed with HIV, wherein the immune cells are activated by contact with a phorbol ester and a calcium ionophore. Methods of using the disclosed compositions are also disclosed.

Abstract: A floor mat clip assembly adapted to attach a floor mat to the interior carpet of a vehicle is disclosed. The clip assembly includes a floor clip attached to the carpet and a mat clip attached to the floor mat. The floor clip includes a base member and a top member having a locking member rotatably attached thereto. The base and top member of the floor clip are adapted to cut through the carpet and interlock to sandwich the carpet therebetween. The mat clip includes a top member and a base member, each having an aperture and adapted to interlock to sandwich the mat therebetween. The mat can then be positioned to insert the locking member of the floor clip through the aperture of the mat clip. A subsequent rotation of the locking member locks together the respective clips to secure the floor mat to the interior carpet.

Abstract: A floor mat clip assembly adapted to attach a floor mat to the interior carpet of a vehicle is disclosed. The clip assembly includes a floor clip attached to the carpet and a mat clip attached to the floor mat. The floor clip includes a base member and a top member having a locking member rotatably attached thereto. The base and top member of the floor clip are adapted to cut through the carpet and interlock to sandwich the carpet therebetween. The mat clip includes a top member and a base member, each having an aperture and adapted to interlock to sandwich the mat therebetween. The mat can then be positioned to insert the locking member of the floor clip through the aperture of the mat clip. A subsequent rotation of the locking member locks together the respective clips to secure the floor mat to the interior carpet.

Abstract: A floor mat clip assembly adapted to attach a floor mat to the interior carpet of a vehicle is disclosed. The clip assembly includes a floor clip attached to the carpet and a mat clip attached to the floor mat. The floor clip includes a base member and a top member having a locking member rotatably attached thereto. The base and top member of the floor clip are adapted to cut through the carpet and interlock to sandwich the carpet therebetween. The mat clip includes a top member and a base member, each having an aperture and adapted to interlock to sandwich the mat therebetween. The mat can then be positioned to insert the locking member of the floor clip through the aperture of the mat clip. A subsequent rotation of the locking member locks together the respective clips to secure the floor mat to the interior carpet.

Abstract: A method includes storing information associated with at least one purchaser in a database. The information may include discount or rate information to be applied when calculating charges for purchases. The method also includes receiving, from a seller, payment card information associated with the sale of fuel to a first purchaser and determining whether the first purchaser is registered in the database. The method also includes calculating, when the first purchaser is registered in the database, a charge amount based on information stored in the database.

Abstract: The respiratory syncytial virus (RSV) is a major cause of lower respiratory tract disease in infants and children throughout the world. RSV is a major cause of pneumonia and bronchiolitis in infants under one year of age, and is a major cause of fatal respiratory tract disease in these infants. The treatment and prevention of RSV infection has been problematic. However, the present invention addresses some of these concerns by providing attenuated RSV strains that are suitable for inclusion in immunizing compositions. Specifically, the present invention is directed toward the introduction of growth restriction mutations into incompletely attenuated host range-restricted cold-passaged respiratory syncytial virus (cpRSV) strains by further passage of the strains at increasingly reduced temperatures to produce derivative strains which are more satisfactorily attenuated.

Abstract: The present invention provides vaccine compositions of attenuated respiratory syncytial virus (RSV). More particularly, the attenuated virus may be a derivative of RSV which has been incompletely attenuated by cold-passage or introduction of mutations which produce virus having a temperature sensitive (ts) or cold adapted (ca) phenotype. The invention also provides methods for stimulating the immune system of an individual to induce protection against respiratory syncytial virus by administration of attenuated RSV. The invention also provides pure cultures of attenuated RS virus, wherein the virus has been more completely attenuated by the further derivatization of previously identified incompletely attenuated ts or cp mutants.

Abstract: The present invention provides vaccine compositions of attenuated respiratory syncytial virus (RSV). More particularly, the attenuated virus may be a derivative of RSV which has been incompletely attenuated by cold-passage or introduction of mutations which produce virus having a temperature sensitive (ts) or cold adapted (ca) phenotype. The invention also provides methods for stimulating the immune system of an individual to induce protection against respiratory syncytial virus by administration of attenuated RSV. The invention also provides pure cultures of attenuated RS virus, wherein the virus has been more completely attenuated by the further derivatization of previously identified incompletely attenuated ts or cp mutants.