Abstract: Compositions and methods for enzymatic decontamination by inactivation of Hazardous agents are provided. Hazardous agents of microbial and chemical origin can be neutralized by H2O2. The methods described herein provide for enzymatic production of H2O2 in situ using oxidoreductase enzymes that use oxygen as an acceptor and their alcohol substrates. The enzymatically produced H2O2 and corresponding aldehydes have potent antimicrobial properties. The enzymatically produced H2O2 also can detoxify chemical agents in situ. The decontaminating power of the oxidoreductase enzymes that use oxygen as an acceptor may be amplified by addition of reagents, such as acetyl donors or base catalysts that, in the presence of H2O2, yield peroxy acid derivatives and hydroperoxy anions. Such derivatives can neutralize biological and chemical agents, thus providing a broadly applicable decontamination method.

Abstract: The present invention is directed to a novel method for analyzing aqueous systems to simultaneously quantify the concentrations of multiple active or inactive treating components which may be contained therein. The method comprises determining the absorbance or emission spectrum of the components in the aqueous system over the wavelength range of from 200 to 2500 nm, and then applying chemometrics algorithms to extract and analyze features in the spectrum that are specific to the qualitative and quantitative concentrations from the specific components to simultaneously determine the concentrations of the components.