Abstract: A cell culture system for the production of cells and cell derived products includes a reusable instrumentation base device incorporating hardware to support cell culture growth. A disposable cultureware module including a cell growth chamber is removably attachable to the instrumentation base device. The base device includes microprocessor control and a pump for circulating cell culture medium through the cell growth chamber. The cultureware module is removably attached to the instrumentation base device. Cells are introduced into the cell growth chamber and a source of medium is fluidly attached to the cultureware module. Operating parameters are programmed into the microprocessor control. The pump is operated to circulate the medium through the cell growth chamber to grow cells or cell products therein. The grown cells or cell products are harvested from the cell growth chamber and the cultureware module is then disposed.

Abstract: A cell culture system for the production of cells and cell derived products includes a reusable instrumentation base device incorporating hardware to support cell culture growth. A disposable cultureware module including a cell growth chamber is removably attachable to the instrumentation base device. The base device includes microprocessor control and a pump for circulating cell culture medium through the cell growth chamber. The cultureware module is removably attached to the instrumentation base device. Cells are introduced into the cell growth chamber and a source of medium is fluidly attached to the cultureware module. Operating parameters are programmed into the microprocessor control. The pump is operated to circulate the medium through the cell growth chamber to grow cells or cell products therein. The grown cells or cell products are harvested from the cell growth chamber and the cultureware module is then disposed.

Abstract: An apparatus and method for culturing cells includes a waste removal and product concentrating loop in which waste is removed from the medium and product produced by the cells is concentrated for harvesting. The apparatus includes a reservoir for retaining a supply of medium. The reservoir is in fluid communication with a cell culturing loop wherein medium flows from the reservoir to the cells and back to the reservoir. The medium waste removal and concentrating loop removes waste components produced by the cells and is in fluid communication with the reservoir such that medium is transferred from the reservoir to the waste removal and concentrating loop and back to the reservoir. The waste removal and concentrating loop includes a device having a semipermeable membrane. The medium flows along one side of the membrane for selective transfer of the waste components through the membrane.