Patents by Inventor Mark D. Pyle

Mark D. Pyle has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • GENOMIC DNA HYBRIDIZATION PROBE AND IMMUNOENZYMATIC COLOR PIGMENT DETECTION ASSAY
    Publication number: 20020058248
    Abstract: A method for detecting the presence of micronuclei in cells of an organism comprises the steps of
    Type: Application
    Filed: March 3, 1999
    Publication date: May 16, 2002
    Inventors: CHRISTOPHER J. KOLANKO, MARK D. PYLE
  • Micronucleus assay with genomic DNA hybridization probe and enzymatic color pigment detection
    Patent number: 6387618
    Abstract: A method for detecting the presence of micronuclei in cells of an organism comprises the steps of (a) isolating cells of the organism, (b) exposing the cells to a hybridization probe, the hybridization probe comprising digested, labeled whole genomic DNA, the digested genomic DNA being labeled with a first binding member capable of specifically binding with a second binding member, whereby, as a result of exposing the cells to the hybridization probe, the hybridization probe binds hybridizes with DNA in the cells, including DNA contained in micronuclei, if present, (c) exposing the cells to a compound comprising the second binding member coupled to an enzyme capable of reacting with a chromogenic substrate to convert the chromogenic substrate into a colored pigment, whereby, as a result of exposing the cells to the compound, the compound binds to the hybridization probe that is hybridized with the DNA in the cells, (d) exposing the cells to the chromogenic substrate, whereby the chromogenic substrate is c
    Type: Grant
    Filed: March 3, 1999
    Date of Patent: May 14, 2002
    Assignee: The United States of America as represented by the Secretary of the Navy
    Inventors: Christopher J. Kolanko, Mark D. Pyle