Abstract: The present invention generally relates to methods for culturing and analyzing cells using liquid bridges.

Abstract: The invention generally relates to an integrated fluidic circuit. In certain embodiments, the circuit includes a main chamber having a withdrawal port, a carrier fluid occupying a volume in the chamber, in which the carrier fluid is immiscible with a sample fluid, and a plurality of liquid bridges disposed within the chamber.

Abstract: A method of processing geophysical data including at least measured potential field data from a potential field survey of a surveyed region of the earth to provide a representation of the geology of said surveyed region, the method comprising generating a first model of said surveyed region by fitting data predicted by said first model to said measured data for a specified frequency range; predicting full range potential field data for all measured frequencies using said generated first model; comparing said full range predicted data to said measured potential field data to provide full range residual data representing a difference between the full range predicted data and the full range measured data, and interpreting said full range residual data to provide a representation of said geology of said surveyed region.

Abstract: The present invention generally relates methods for analyzing agricultural and/or environmental samples using liquid bridges. In certain embodiments, the invention provides a method for analyzing an agricultural sample for a desired trait including obtaining a gene or gene product from an agricultural sample, in which the gene or gene product is in a first fluid; providing a liquid bridge for mixing the gene or gene product with at least one reagent to form a mixed droplet that is wrapped in an immiscible second fluid; and analyzing the mixed droplet to detect a desired trait of the agricultural sample.

Abstract: We describe a method of processing geophysical data including at least measured potential field data from a potential field survey of a surveyed region of the earth to provide a three-dimensional representation of the underlying geology of said surveyed region, the method comprising: inputting terrain-corrected potential field data for said surveyed region, said potential field data comprising data for a range of spatial wavelengths, geological features at different depths in said surveyed region being associated with different wavelengths in said range of wavelengths; filtering said potential field data by spatial wavelength to generate a first plurality of filtered sets of potential field data, each relating to a respective wavelength or range of wavelengths, each targeting geological features at a different respective said depth; processing each said filtered set of potential field data, to identify a set of spatial features comprising one or both of line spatial features and point spatial features in each

Abstract: The invention provides a lubricating composition containing an oil of lubricating viscosity, an oil soluble molybdenum compound, and an ashless antiwear agent. The invention further provides for a new antioxidant. The lubricating composition is suitable for lubricating an internal combustion engine.

Abstract: The present invention relates to a method of lubricating an aluminium-composite surface by supplying to the aluminium composite surface (typically an internal combustion engine aluminium surface) a lubricating composition comprising an oil of lubricating viscosity and an ashless antiwear agent.

Abstract: A composition comprising a mixture of: (i) an aromatic polycarbonate; (ii) a graft copolymer including polyacrylonitrile; and, (iii) a non-crosslinked acrylic polymer having a weight average molecular weight (Mw) of less than or equal to 65,000 Daltons (Da).

Abstract: The invention provides a lubricating composition containing an oil of lubricating viscosity and a hydrogenated copolymer of an olefin block and vinyl aromatic block, wherein the copolymer is optionally functionalised. The invention further provides a method for preparing a hydrogenated copolymer; and the use of the lubricating composition.

Abstract: The present invention generally relates to systems and methods for mixing and dispensing a sample droplet from a microfluidic system, such as a liquid bridge system. In certain embodiments, the invention provides systems for mixing and dispensing sample droplets, including a sample acquisition stage, a device for mixing sample droplets to form sample droplets wrapped in an immiscible carrier fluid, a dispensing port, and at least one channel connecting the stage, the droplet mixing device, and the port, in which the system is configured to establish a siphoning effect for dispensing the droplets.

Abstract: A microfluidic connector (1) comprises an enclosure (6, 7), a fluidic inlet port (2) and a fluidic outlet port (3), in the enclosure, in which the inlet and outlet ports (2, 3) are movable with respect to each other, for example, mutual spacing between the inlet and outlet ports (2, 3) is variable. A port (2) is in a fixed part (6) of the enclosure, and another port (3) is in a part (7) of the enclosure which slides with respect to the fixed part. There may be multiple inlet ports (22, 23) and/or multiple outlet ports (24, 25). Also, there may be an auxiliary port (45) for introduction of fluid into the enclosure (47, 48) or removal of fluid from the enclosure.

Abstract: The invention provides methods of conducting a nucleic acid reaction, including methods for performing digital PCR using a “droplet-in-oil” technology. In the methods, the starting sampled is segmented at least partially into a set of sample droplets each containing on average about one or fewer copies of a target nucleic acid. The droplets are passed in a continuous flow of immiscible carrier fluid through a channel that passes through a thermal cycler, whereby the target is amplified. In one implementation, the droplets are about 350 nl each and the number of positively amplified droplets is counted at the near-saturation point.

Abstract: A microfluidic analysis system (1) performs polymerase chain reaction (PCR) analysis on a bio sample. In a centrifuge (6) the sample is separated into DNA and RNA constituents. The vortex is created by opposing flow of a silicon oil primary carrier fluid effecting circulation by viscous drag. The bio sample exits the centrifuge enveloped in the primary carrier fluid. This is pumped by a flow controller (7) to a thermal stage (9). The thermal stage (9) has a number of microfluidic devices (70) each having thermal zones (71, 72, 73) in which the bio sample is heated or cooled by heat conduction to/from a thermal carrier fluid and the primary carrier fluid. Thus, the carrier fluids envelope the sample, control its flowrate, and control its temperature without need for moving parts at the micro scale.

Abstract: A real time detector system for monitoring ram alignment in a can bodymaker measures ram (10) position immediately before and during impact with the dome forming station (1). Displacement measurements of the ram enable the user to adjust dome (1) position or otherwise correct ram (10) alignment and avoid multiple can failures.

Abstract: An apparatus (1) is for DNA amplification with quantitative measurements. A biological sample is held in a cell (2) for the amplification, the cell (2) defining a single space within which the sample rotates. On one side a copper heater (3) is located to supply heat to the cell (2), and on the other side there is a cooling copper block (4) withdrawing heat from the cell. The locations of the heater (3) and the cooling block (4) generate a natural convection loop internally within the cell (2) without need for active cooling—the block (4) passively cooling by withdrawing heat from the direction of the heater (3). A detector (9, 27) captures readings in real time and a processor (10) generates an S-curve for change of sample emission with time. The S-curve (FIGS. 4 and 5) also includes a thermal cycle number corresponding to the time parameter, so that the S-curve is given in the traditional qPCR intensity vs. cycle number.

Abstract: Measurement of gene expression relative to an endogenous control gene is prone to excessive variability between samples and even replicates. The disclosure provides methods for normalizing expression levels of a gene by scaling gene expression levels to that of the most highly expressed gene in the set of genes whose expression levels are measured, rather than a house-keeping gene.

Abstract: A purge system for use with a well intervention system and apparatus is disclosed. The purge system permits well fluids contained in a subsea intervention system to be purged prior to retrieval of the intervention system to the surface. The system comprises a vessel (44) for storing and deploying wireline tooling, the vessel being exposed to well fluids via the well control package (16) and first fluid communication means (58) connected between a purging fluid supply and the well control package, and second fluid communication means (60) connected between the vessel and the well control package wherein, in use, the purging fluid supply to the vessel via the first fluid communication means displaces fluid from the vessel into a second fluid communication means and into the well control package.

Abstract: The present invention generally relates to methods of constructing liquid bridges and methods of forming predetermined combinations of samples using liquid bridges.

Abstract: A multi-port liquid bridge (1) adds aqueous phase droplets (10) in an enveloping oil phase carrier liquid (11) to a draft channel (4, 6). A chamber (3) links four ports, and it is permanently full of oil (11) when in use. Oil phase is fed in a draft flow from an inlet port (4) and exits through a draft exit port (6) and a compensating flow port (7). The oil carrier and the sample droplets (3) (“aqueous phase”) flow through the inlet port (5) with an equivalent fluid flow subtracted through the compensating port (7). The ports of the bridge (1) are formed by the ends of capillaries held in position in plastics housings. The phases are density matched to create an environment where gravitational forces are negligible. This results in droplets (10) adopting spherical forms when suspended from capillary tube tips. Furthermore, the equality of mass flow is equal to the equality of volume flow.

Abstract: A microfluidic analysis system (1) performs polymerase chain reaction (PCR) analysis on a bio sample. In a centrifuge (6) the sample is separated into DNA and RNA constituents. The vortex is created by opposing flow of a silicon oil primary carrier fluid effecting circulation by viscous drag. The bio sample exits the centrifuge enveloped in the primary carrier fluid. This is pumped by a flow controller (7) to a thermal stage (9). The thermal stage (9) has a number of microfluidic devices (70) each having thermal zones (71, 72, 73) in which the bio sample is heated or cooled by heat conduction to/from a thermal carrier fluid and the primary carrier fluid. Thus, the carrier fluids envelope the sample, control its flowrate, and control its temperature without need for moving parts at the micro scale.