Abstract: The present invention relates to pluripotent stems cells restricted in their capability to differentiate into cell lineages different from Dopaminergic neuron progenitor cells and derivatives thereof by specific gene knockouts. In particular, the present invention relates to dopaminergic neuron progenitor cells or cell derivatives thereof obtained from these lineage-restricted pluripotent stem cells and uses thereof.

Abstract: The present invention relates to methods of inducing differentiation of stem cells into a specific cell lineage, preferably lung cells. In particular, the invention relates to in vitro methods of inducing differentiation of stem cells into a specific cell lineage. The invention also relates to methods of producing and recovering differentiated stem cells of a specific cell lineage. The invention also includes differentiated stem cells and cell lineages produced by the methods of the present invention. In one aspect of the present invention there is provided a method of inducing differentiation of a stem cell into a specific cell lineage, preferably lung cells, the method including: culturing a stem cell in vitro in the presence of a tissue sample and/or extracellular medium of a tissue sample, under conditions that induce differentiation of the stem cell into a specific cell lineage, wherein the differentiated stem cell is the same cell type as the tissue sample.