Abstract: Conventional expanded microscopy (ExM) is improved upon to show reliable and reproducible 4-fold and 12-fold modified expansion microscopy (mExM). Kits and methods are disclosed to achieve these improvements. The kits can include a sealable mold and instructions for using it in the improved methods. The kits can include a protein digestion buffer including sodium dodecyl sulfate and instructions for using the buffer in the improved methods. The improved methods can include a simplified approach to executing oxygen-sensitive reactions within the methods. The improved methods can include improved protein digestion chemistries, which can facilitate the improvements in reliability and reproducibility.

Abstract: Conventional expanded microscopy (ExM) is improved upon to show reliable and reproducible 4-fold and 12-fold modified expansion microscopy (mExM). Kits and methods are disclosed to achieve these improvements. The kits can include a sealable mold and instructions for using it in the improved methods. The kits can include a protein digestion buffer including sodium dodecyl sulfate and instructions for using the buffer in the improved methods. The improved methods can include a simplified approach to executing oxygen-sensitive reactions within the methods. The improved methods can include improved protein digestion chemistries, which can facilitate the improvements in reliability and reproducibility.

Abstract: Constructs, vectors, and methods for enhancing homology directed repair using the CRISPR/Cas9 gene editing platform are disclosed.

Abstract: A device is provided for the administration of a compound in a body. The device includes a capsule having a reservoir therein for receiving the compound therein and a delivery instrument for positioning the capsule within the body. The delivery instrument includes a barrel having an interior adapted to receive the capsule therein and a dispersal element interacting with the barrel to selectively expel the capsule therefrom and into the body. A diffusion regulator communicates with the reservoir and controls the diffusion of the compound from the reservoir into the body. A retention structure projects from capsule and is configured to retain the capsule at a desired location within the body. Multiple devices may be used in conjunction to provide a multiplexed point source to administer multiple compounds simultaneously in vivo.

Abstract: A device is provided for the administration of a compound in a body. The device includes a capsule having a reservoir therein for receiving the compound therein and a delivery instrument for positioning the capsule within the body. The delivery instrument includes a barrel having an interior adapted to receive the capsule therein and a dispersal element interacting with the barrel to selectively expel the capsule therefrom and into the body. A diffusion regulator communicates with the reservoir and controls the diffusion of the compound from the reservoir into the body. A retention structure projects from capsule and is configured to retain the capsule at a desired location within the body. Multiple devices may be used in conjunction to provide a multiplexed point source to administer multiple compounds simultaneously in vivo.

Abstract: A method is disclosed for the treatment of right to left shunt. The method comprises introducing, into the blood circulation of an individual, to be treated, a therapeutically effective amount of the stabilized microbubbles. In a preferred embodiment, oxygen is additionally administered to the individual.

Abstract: Disclosed are methods of using stabilized microbubbles, such as those formulated from slowly permeating gas, to deliver or remove from tissue at least one gas selected from a respiratory gas, anesthetic gas, inert gas, or toxic gas. The methods comprise introducing, into the blood circulation of an individual to be treated, a therapeutically effective amount of the stabilized microbubbles. The methods are based on using the inherent physical properties of the blood circulation, of the microbubbles, and of the gases. The methods are especially useful for enhancing transport of oxygen in applications or conditions such as blood loss, anemia, organ perfusion, coronary Angioplasty, venous to arterial shunting of blood, oxygenation of ischemic tissues resulting from vascular obstructions, and oxygenation of solid tumor tissues in anticancer therapy.