Abstract: A system for optical imaging of a thick specimen that permits rapid acquisition of data necessary for tomographic reconstruction of the three-dimensional (3D) image. One method involves the scanning of the focal plane of an imaging system and integrating the range of focal planes onto a detector. The focal plane of an optical imaging system is scanned along the axis perpendicular to said plane through the thickness of a specimen during a single detector exposure. Secondly, methods for reducing light scatter when using illumination point sources are presented. Both approaches yield shadowgrams. This process is repeated from multiple perspectives, either in series using a single illumination/detection subsystem, or in parallel using several illumination/detection subsystems. A set of pseudo-projections is generated, which are input to a three dimensional tomographic image reconstruction algorithm.

Abstract: An apparatus and method for providing image acquisition and/or image display in a limited region of interest (ROI). The apparatus comprises a micro-electro-mechanical system (MEMS), preferably integrating a light source, a cantilever, a lens, an actuator, a light detector, and a position sensor. The light source provides light for illuminating the ROI, displaying an image, providing a therapy, and/or performing other functions. The cantilever comprises a resin waveguide with a fixed end attached to a substrate that supports many or all other components. A free end of the cantilever is released from the substrate during fabrication and includes the lens. The actuator scans the free end in orthogonal directions to illuminate the ROI or display an image. The position sensors detect the position of the free end for control. The light detector receives light backscattered from the ROI separate from, or at the fixed end of the cantilever.

Abstract: A method for 3D imaging of cells in an optical tomography system includes moving a biological object relatively to a microscope objective to present varying angles of view. The biological object is illuminated with radiation having a spectral bandwidth limited to wavelengths between 150 nm and 390 nm. Radiation transmitted through the biological object and the microscope objective is sensed with a camera from a plurality of differing view angles. A plurality of pseudoprojections of the biological object from the sensed radiation is formed and the plurality of pseudoprojections is reconstructed to form a 3D image of the cell.

Abstract: An apparatus and method for providing image acquisition and/or image display in a limited region of interest (ROI). The apparatus comprises a micro electro-mechanical system (MEMS), preferably integrating a light source, a cantilever, a lens, an actuator, a light detector, and a position sensor. The light source provides light for illuminating the ROI, displaying an image, providing a therapy, and/or performing other functions. The cantilever comprises a resin waveguide with a fixed end attached to a substrate that supports many or all other components. A free end of the cantilever is released from the substrate during fabrication and includes the lens. The actuator scans the free end in orthogonal directions to illuminate the ROI or display an image. The position sensors detect the position of the free end for control. The light detector receives light backscattered from the ROI separate from, or at the fixed end the cantilever.

Abstract: A method for reconstructing three-dimensional (3D) tomographic images. A set of pseudo-projection images of an object is acquired. Error corrections are applied to the set of pseudo-projection images to produce a set of corrected pseudo-projection images. The set of corrected pseudo-projection images are processed to produce (3D) tomographic images.

Abstract: An optical fiber having a reduced cross-sectional region adjacent to its distal end, which is fused to an optical component, is vibrated, rotating the optical component to scan a region. The optical component has a back focal point that is substantially coincident with an effective light source of the optical fiber, so that the light emanating from the optical component is either substantially collimated or convergent. The optical component is either a ball lens, a drum lens, a graded index lens, or a diffractive optical element. A vibratory node is also made substantially coincident with the back focal point of the optical component, producing a compact scanner with extensive field of view. The optical fiber is preferably reduced in cross-sectional area after the optical component is fused to the optical fiber, by immersion in a three-layer etch apparatus having an etch-stop layer, an etch layer, and a solvent layer.

Abstract: A process includes coating a cylinder having an inner wall and a cylinder axis with a gel coating on the inner wall. Then a specimen mixture including solvent is made to flow through the cylinder while the cylinder is being continuously rotated. The specimen mixture is initially directed to flow along the cylinder axis and such that specimen particles from the specimen mixture are accelerated off the cylinder axis toward the inner wall, so as to form a film of specimen particles embedded into the gel coating.

Abstract: An optical fiber having a reduced cross-sectional region adjacent to its distal end, which is fused to an optical component, is vibrated, rotating the optical component to scan a region. The optical component has a back focal point that is substantially coincident with an effective light source of the optical fiber, so that the light emanating from the optical component is either substantially collimated or convergent. The optical component is either a ball lens, a drum lens, a graded index lens, or a diffractive optical element. A vibratory node is also made substantially coincident with the back focal point of the optical component, producing a compact scanner with extensive field of view. The optical fiber is preferably reduced in cross-sectional area after the optical component is fused to the optical fiber, by immersion in a three-layer etch apparatus having an etch-stop layer, an etch layer, and a solvent layer.

Abstract: Small, rugged scanners micro-fabricated from commercial optical fibers to form waveguides or other structures. The scanning waveguide has a distal portion on which is formed a non-linear taper with a diameter that decreases toward a distal end. Optionally, a hinge portion having a reduced diameter can be formed in the distal portion, improving the scanning properties of the waveguide. A micro-lens can be integrally formed at the distal tip of the waveguide with either a droplet of an optical adhesive, or by using an energy beam to melt the material of the waveguide to form a droplet. The droplet is shaped with an externally applied force. When mechanically driven in vibratory resonance, the tip of the optical waveguides moves in linear or two-dimensional scan patterns of relatively high amplitude and frequency, and large field of view. The scanner can be used either for image acquisition or image display.

Abstract: An optical fiber having a reduced cross-sectional region adjacent to its distal end, which is fused to an optical component, is vibrated, rotating the optical component to scan a region. The optical component has a back focal point that is substantially coincident with an effective light source of the optical fiber, so that the light emanating from the optical component is either substantially collimated or convergent. The optical component is either a ball lens, a drum lens, a graded index lens, or a diffractive optical element. A vibratory node is also made substantially coincident with the back focal point of the optical component, producing a compact scanner with extensive field of view. The optical fiber is preferably reduced in cross-sectional area after the optical component is fused to the optical fiber, by immersion in a three-layer etch apparatus having an etch-stop layer, an etch layer, and a solvent layer.

Abstract: An apparatus and method for providing image acquisition and/or image display in a limited region of interest (ROI). The apparatus comprises a micro electromechanical system (MEMS), preferably integrating a light source, a cantilever, a lens, an actuator, a light detector, and a position sensor. The light source provides light for illuminating the ROI, displaying an image, providing a therapy, and/or performing other functions. The cantilever comprises a resin waveguide with a fixed end attached to a substrate that supports many or all other components. A free end of the cantilever is released from the substrate during fabrication and includes the lens. The actuator scans the free end in orthogonal directions to illuminate the ROI or display an image. The position sensors detect the position of the free end for control. The light detector receives light backscattered from the ROI separate from, or at the fixed end the cantilever.

Abstract: A system for optical imaging of a thick specimen that permits rapid acquisition of data necessary for tomographic reconstruction of the three-dimensional (3D) image. One method involves the scanning of the focal plane of an imaging system and integrating the range of focal planes onto a detector. The focal plane of an optical imaging system is scanned along the axis perpendicular to said plane through the thickness of a specimen during a single detector exposure. Secondly, methods for reducing light scatter when using illumination point sources are presented. Both approaches yield shadowgrams. This process is repeated from multiple perspectives, either in series using a single illumination/detection subsystem, or in parallel using several illumination/detection subsystems. A set of pseudo-projections is generated, which are input to a three dimensional tomographic image reconstruction algorithm.

Abstract: Small, rugged scanners micro-fabricated from commercial optical fibers to form waveguides or other structures. The scanning waveguide has a distal portion on which is formed a non-linear taper with a diameter that decreases toward a distal end. Optionally, a hinge portion having a reduced diameter can be formed in the distal portion, improving the scanning properties of the waveguide. A micro-lens can be integrally formed at the distal tip of the waveguide with either a droplet of an optical adhesive, or by using an energy beam to melt the material of the waveguide to form a droplet. The droplet is shaped with an externally applied force. When mechanically driven in vibratory resonance, the tip of the optical waveguides moves in linear or two-dimensional scan patterns of relatively high amplitude and frequency, and large field of view. The scanner can be used either for image acquisition or image display.