Abstract: A bioreactor apparatus and cell culturing system is provided for the automated cultivation and processing of living cells remotely both on earth and in low gravity which utilizes a generally cylindrical reactor vessel that may be optionally rotated about its cylindrical axis while allowing the entrance of fresh or recycled fluid and the removal, optionally, of spent medium, medium to be recycled or filtered or unfiltered medium for the collection of samples. A method of exchanging gases between the culture medium and ambient gases is provided. A fresh-medium storage bag and peristaltic pump is used for batch feeding, perfusion or sample collection. An enclosure and manifold representing an additional level of chemical containment and a series of pinch valves for the periodic collection of samples of suspended cells or cell-free medium is disposed therein together with a humidity control system.

Abstract: A bioreactor apparatus and cell culturing system is provided for the automated cultivation and processing of living cells remotely both on earth and in low gravity which utilizes a generally cylindrical reactor vessel that may be optionally rotated about its cylindrical axis while allowing the entrance of fresh or recycled fluid and the removal, optionally, of spent medium, medium to be recycled or filtered or unfiltered medium for the collection of samples. The bioreactor vessel includes a cylindrical wall, two cover plates, two rotary unions, fill ports, and a polymeric filter. A method of exchanging gases between the culture medium and ambient gases is fabricated from a user-selected length of permeable tubing and a peristaltic pump. A polymeric fresh-medium storage bag and peristaltic pump is used for batch feeding, perfusion or sample collection.

Abstract: A dynamic prosthetic vertebral body for use in a cavity left by resection of a natural vertebral body and adjacent intervertebral discs. The prosthesis includes a pair of rigid housings joined by a connecting structure that is operable to shift the housings apart into supportive engagement with the healthy vertebral bodies adjacent the site of the resected body. Anchoring pins project outwardly in axial directions from the respective housings for secure fixation to the healthy vertebral bodies. Within the chamber of each housing is a rigid suspension plate surrounded by an elastomeric suspension medium, and the suspension plates of the two housings are joined by the expandable connecting structure. The relative sizes of the suspension plates and the chambers in which they are received allow limited movement of the housings, resulting in a prosthesis that provides the required support but also mimics the dynamics of a normal vertebra and adjacent discs.