Patents by Inventor Mark Eshoo
Mark Eshoo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20250049360Abstract: A lactate-responsive enzyme may form the basis for lactate detection and quantification using an electrochemical analyte sensor. Various features may be incorporated within an analyte sensor containing a lactate-responsive enzyme, particularly lactate oxidase, to improve sensitivity and response stability of the analyte sensor. Such analyte sensors may comprise: a working electrode having an active area disposed thereon, and a mass transport limiting membrane overcoating at least the active area upon the working electrode. The active area comprises at least a polymer, an albumin, and a lactate-responsive enzyme that is covalently bonded to the polymer. The mass transport limiting membrane may comprise at least a crosslinked polyvinylpyridine homopolymer or copolymer. The analyte sensors may determine a lactate concentration in a biological fluid, particularly in vivo, which may be correlated to various physiological conditions.Type: ApplicationFiled: July 22, 2024Publication date: February 13, 2025Applicant: ABBOTT DIABETES CARE INC.Inventors: Kuan-Chou CHEN, Tianmei OUYANG, Stephen OJA, Benjamin J. FELDMAN, Hyun CHO, Lam TRAN, Mark ESHOO
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Patent number: 12076145Abstract: A lactate-responsive enzyme may form the basis for lactate detection and quantification using an electrochemical analyte sensor. Various features may be incorporated within an analyte sensor containing a lactate-responsive enzyme, particularly lactate oxidase, to improve sensitivity and response stability of the analyte sensor. Such analyte sensors may comprise: a working electrode having an active area disposed thereon, and a mass transport limiting membrane overcoating at least the active area upon the working electrode. The active area comprises at least a polymer, an albumin, and a lactate-responsive enzyme that is covalently bonded to the polymer. The mass transport limiting membrane may comprise at least a crosslinked polyvinylpyridine homopolymer or copolymer. The analyte sensors may determine a lactate concentration in a biological fluid, particularly in vivo, which may be correlated to various physiological conditions.Type: GrantFiled: January 28, 2019Date of Patent: September 3, 2024Assignee: Abbott Diabetes Care Inc.Inventors: Kuan-Chou Chen, Tianmei Ouyang, Stephen Oja, Benjamin Feldman, Hyun Cho, Lam Tran, Mark Eshoo
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Publication number: 20220338763Abstract: In vivo monitoring devices and systems for enzymes and/or analytes including devices having a reactant reservoir are provided.Type: ApplicationFiled: July 11, 2022Publication date: October 27, 2022Inventors: Mark ESHOO, Benjamin FELDMAN, Tianmei OUYANG, Lam TRAN
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Patent number: 11432750Abstract: In vivo monitoring devices and systems for enzymes and/or analytes including devices having a reactant reservoir are provided.Type: GrantFiled: March 13, 2017Date of Patent: September 6, 2022Assignee: Abbott Diabetes Care Inc.Inventors: Mark Eshoo, Benjamin Feldman, Tianmei Ouyang, Lam Tran
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Publication number: 20210214779Abstract: A method for estimating a diagnostic score includes adding a first aliquot of a test sample to a first reaction vessel, and a second aliquot of the test sample to a second reaction vessel. The test sample includes a first target nucleic acid and a second target nucleic acid. The second target nucleic acid has a lower expected abundance than the first target nucleic acid. The second aliquot has a larger volume than the first aliquot. A first relative abundance value of the first target nucleic acid and a second relative abundance value of the second target nucleic acid are estimated by performing a first real-time quantitative isothermal amplification assay in the first reaction vessel, and performing a second real-time quantitative isothermal amplification assay in the second reaction vessel, respectively. The diagnostic score is estimated based on the first relative abundance value and the second relative abundance value.Type: ApplicationFiled: September 18, 2019Publication date: July 15, 2021Inventors: David Rawling, Timothy E. Sweeney, Mark Eshoo
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Publication number: 20190320947Abstract: A lactate-responsive enzyme may form the basis for lactate detection and quantification using an electrochemical analyte sensor. Various features may be incorporated within an analyte sensor containing a lactate-responsive enzyme, particularly lactate oxidase, to improve sensitivity and response stability of the analyte sensor. Such analyte sensors may comprise: a working electrode having an active area disposed thereon, and a mass transport limiting membrane overcoating at least the active area upon the working electrode. The active area comprises at least a polymer, an albumin, and a lactate-responsive enzyme that is covalently bonded to the polymer. The mass transport limiting membrane may comprise at least a crosslinked polyvinylpyridine homopolymer or copolymer. The analyte sensors may determine a lactate concentration in a biological fluid, particularly in vivo, which may be correlated to various physiological conditions.Type: ApplicationFiled: January 28, 2019Publication date: October 24, 2019Applicant: Abbott Diabetes Care Inc.Inventors: Kuan-Chou Chen, Tianmei Ouyang, Stephen Oja, Benjamin Feldman, Hyun Cho, Lam Tran, Mark Eshoo
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Publication number: 20180094288Abstract: The present invention provides compositions, kits, and methods for synthesizing polyadenylic acid using polynucleotide phosphorylase, adenosine diphosphate, a buffering agent, and a divalent metal cation. In certain embodiments, the adenosine diphosphate is present at a concentration between about 5.0 mM and about 100 mM, and the buffering agent has a pH around 8.0. In some embodiments, the prepared polyadenylic acid compositions are free of detectable nucleic acid.Type: ApplicationFiled: November 22, 2017Publication date: April 5, 2018Inventors: Mark Eshoo, Curtis Phillipson
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Patent number: 9914965Abstract: The present invention provides compositions and methods for rapidly amplifying target nucleic acid (e.g., using whole genome amplification) that allows small amounts of starting nucleic acid to be employed. In certain embodiments, the methods employ compositions that comprise: phi29 polymerase, exo? Klenow polymerase and/or Klenow polymerase, dNTPs, primers, and a buffering agent. In some embodiments, the target nucleic acid is amplified at a rate that would result in at least 1000-fold amplification in thirty minutes.Type: GrantFiled: August 29, 2016Date of Patent: March 13, 2018Assignee: IBIS BIOSCIENCES, INC.Inventors: Mark Eshoo, Stanley Motley, John Picuri
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Publication number: 20170266659Abstract: The present invention provides integrated sample preparation systems and stabilized enzyme mixtures. In particular, the present invention provides microfluidic cards configured for processing a sample and generating DNA libraries that are suitable for use in sequencing methods (e.g., next generation sequencing methods) or other suitable nucleic acid analysis methods. The present invention also provides stabilized enzyme mixtures containing an enzyme (e.g., an enzyme used in whole genome amplification), BSA, and a sugar. Such enzyme mixtures may be lyophilized and stored at room temperature without significant loss of enzyme activity for months.Type: ApplicationFiled: March 30, 2017Publication date: September 21, 2017Inventors: Mark Eshoo, John Picuri
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Publication number: 20170258378Abstract: In vivo monitoring devices and systems for enzymes and/or analytes including devices having a reactant reservoir are provided.Type: ApplicationFiled: March 13, 2017Publication date: September 14, 2017Inventors: Mark Eshoo, Benjamin Feldman, Tianmei Ouyang, Lam Tran
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Patent number: 9737887Abstract: The present invention provides integrated sample preparation systems and stabilized enzyme mixtures. In particular, the present invention provides microfluidic cards configured for processing a sample and generating DNA libraries that are suitable for use in sequencing methods (e.g., next generation sequencing methods) or other suitable nucleic acid analysis methods. The present invention also provides stabilized enzyme mixtures containing an enzyme (e.g., an enzyme used in whole genome amplification), BSA, and a sugar. Such enzyme mixtures may be lyophilized and stored at room temperature without significant loss of enzyme activity for months.Type: GrantFiled: February 23, 2015Date of Patent: August 22, 2017Assignee: IBIS BIOSCIENCES, INC.Inventors: Mark Eshoo, John Picuri
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Publication number: 20170067089Abstract: The present invention provides compositions and methods for rapidly amplifying target nucleic acid (e.g., using whole genome amplification) that allows small amounts of starting nucleic acid to be employed. In certain embodiments, the methods employ compositions that comprise: phi29 polymerase, exo-Klenow polymerase and/or Klenow polymerase, dNTPs, primers, and a buffering agent. In some embodiments, the target nucleic acid is amplified at a rate that would result in at least 1000-fold amplification in thirty minutes.Type: ApplicationFiled: August 29, 2016Publication date: March 9, 2017Inventors: Mark Eshoo, Stanley Motley, John Picuri
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Patent number: 9428801Abstract: The present invention provides compositions and methods for rapidly amplifying target nucleic acid (e.g., using whole genome amplification) that allows small amounts of starting nucleic acid to be employed. In certain embodiments, the methods employ compositions that comprise: phi29 polymerase, exo- Klenow polymerase and/or Klenow polymerase, dNTPs, primers, and a buffering agent. In some embodiments, the target nucleic acid is amplified at a rate that would result in at least 1000-fold amplification in thirty minutes.Type: GrantFiled: December 29, 2011Date of Patent: August 30, 2016Assignee: IBIS BIOSCIENCES, INC.Inventors: Mark Eshoo, Stanley Motley, John Picuri
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Publication number: 20160186241Abstract: Provided herein are buffers for the stabilization of nucleic acid molecules. The buffers find particular use for the stabilization of trace amounts of nucleic acid molecules in a variety of environments, including repeated freeze/thaw cycles. For example, in some embodiments, provided herein are compositions comprising tris(hydroxymethyl)aminomethane (Tris), ethylenediaminetetraacetic acid (EDTA), polyadenylic acid, and a synthetic DNA oligonucleotide.Type: ApplicationFiled: March 7, 2016Publication date: June 30, 2016Inventors: Mark Eshoo, David D. Duncan
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Publication number: 20160051959Abstract: The present invention provides compositions and methods for preparing a nucleic acid library in a multi-purpose buffer (e.g., employing whole genome amplification), where nucleic acid purification is not required between or during steps. In certain embodiments, small amounts of starting nucleic acid (e.g., genomic DNA) are employed and the steps are accomplished in a single container. In some embodiments, the nucleic acid library is subjected to sequencing methodologies or rolling circle amplification.Type: ApplicationFiled: August 17, 2015Publication date: February 25, 2016Inventors: Mark Eshoo, John Picuri, Stanley Motley
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Publication number: 20150231629Abstract: The present invention provides integrated sample preparation systems and stabilized enzyme mixtures. In particular, the present invention provides microfluidic cards configured for processing a sample and generating DNA libraries that are suitable for use in sequencing methods (e.g., next generation sequencing methods) or other suitable nucleic acid analysis methods. The present invention also provides stabilized enzyme mixtures containing an enzyme (e.g., an enzyme used in whole genome amplification), BSA, and a sugar. Such enzyme mixtures may be lyophilized and stored at room temperature without significant loss of enzyme activity for months.Type: ApplicationFiled: February 23, 2015Publication date: August 20, 2015Inventors: Mark Eshoo, John Picuri
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Patent number: 9109222Abstract: The present invention provides compositions and methods for preparing a nucleic acid library in a multi-purpose buffer (e.g., employing whole genome amplification), where nucleic acid purification is not required between or during steps. In certain embodiments, small amounts of starting nucleic acid (e.g., genomic DNA) are employed and the steps are accomplished in a single container. In some embodiments, the nucleic acid library is subjected to sequencing methodologies or rolling circle amplification.Type: GrantFiled: December 27, 2011Date of Patent: August 18, 2015Assignee: IBIS BIOSCIENCES, INC.Inventors: Mark Eshoo, John Picuri, Stanley Motley
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Patent number: 8961899Abstract: The present invention provides integrated sample preparation systems and stabilized enzyme mixtures. In particular, the present invention provides microfluidic cards configured for processing a sample and generating DNA libraries that are suitable for use in sequencing methods (e.g., next generation sequencing methods) or other suitable nucleic acid analysis methods. The present invention also provides stabilized enzyme mixtures containing an enzyme (e.g., an enzyme used in whole genome amplification), BSA, and a sugar. Such enzyme mixtures may be lyophilized and stored at room temperature without significant loss of enzyme activity for months.Type: GrantFiled: June 24, 2013Date of Patent: February 24, 2015Assignee: IBIS Biosciences, Inc.Inventors: Mark Eshoo, John Picuri
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Publication number: 20130274147Abstract: The present invention provides integrated sample preparation systems and stabilized enzyme mixtures. In particular, the present invention provides microfluidic cards configured for processing a sample and generating DNA libraries that are suitable for use in sequencing methods (e.g., next generation sequencing methods) or other suitable nucleic acid analysis methods. The present invention also provides stabilized enzyme mixtures containing an enzyme (e.g., an enzyme used in whole genome amplification), BSA, and a sugar. Such enzyme mixtures may be lyophilized and stored at room temperature without significant loss of enzyme activity for months.Type: ApplicationFiled: June 24, 2013Publication date: October 17, 2013Applicant: IBIS BIOSCIENCES, INC.Inventors: Mark Eshoo, John Picuri
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Publication number: 20120171726Abstract: The present invention provides compositions and methods for rapidly amplifying target nucleic acid (e.g., using whole genome amplification) that allows small amounts of starting nucleic acid to be employed. In certain embodiments, the methods employ compositions that comprise: phi29 polymerase, exo- Klenow polymerase and/or Klenow polymerase, dNTPs, primers, and a buffering agent. In some embodiments, the target nucleic acid is amplified at a rate that would result in at least 1000-fold amplification in thirty minutes.Type: ApplicationFiled: December 29, 2011Publication date: July 5, 2012Applicant: IBIS BIOSCIENCES, INC.Inventors: Mark Eshoo, Stanley Motley, John Picuri