Abstract: Methods of detecting and/or quantitating low-abundance proteins in biological samples using size-exclusion chromatography and mass spectrometry. The methods can include subjecting a biological sample comprising a target protein to size-exclusion chromatography, obtaining from the size-exclusion chromatography a size-exclusion chromatography sample comprising the target protein, preparing from the size-exclusion chromatography sample a mass-spectrometry sample comprising the target protein or a fragment thereof, and detecting the target protein or the fragment thereof in the mass-spectrometry sample using mass spectrometry. The target protein can comprise MUC16, among other proteins, and the biological sample can comprise a blood sample, such as plasma or serum.

Abstract: The present invention concerns methods and compositions that involve whey protein isolates. Methods of the invention include purification processes for preparing whey proteins that are substantially nondenatured across a range of pH, including their isoelectric points. As such, they have low turbidity in solution across a wide range of pH values. Whey protein isolates can be integrated into compositions and solutions that have nutritional, pharmaceutical, and other applications.

Abstract: It is disclosed here that a protein with a heat-labile specific binding activity can be stabilized with a saccharide compound with regard to the binding activity. To heat-stabilize the protein, it is mixed with a saccharide compound in a liquid suspension and the suspension is then dried to produce a solid that contains the protein and the saccharide. The saccharide compounds that possess the protein heat-stabilization activity include monosaccharides, disaccharides, polysaccharides, alkylated monosaccharides, alkylated disaccharides, alkylated polysaccharides, monosaccharide alcohols and alkylated monosaccharide alcohols.