Abstract: The embodiments include an array in intimate adjacent contact with a substrate foundation. The array has a plurality of radio frequency (RF) witness films overlain on the substrate foundation. Each RF witness film is a unit cell defined in a three-dimensional coordinate frame of reference, and is centered at an origin of the three-dimensional coordinate frame of reference. Each RF witness film in the plurality of RF witness films is equally-spaced from adjacent RF witness films.

Abstract: In one aspect, the invention relates to an immunogenic composition that includes a mutant Clostridium difficile toxin A and/or a mutant Clostridium difficile toxin B. The mutant toxin may include a glucosyltransferase domain having at least one mutation and a cysteine protease domain having at least one mutation, relative to the corresponding wild-type C. difficile toxin. The mutant toxins may include at least one amino acid that is chemically crosslinked. In another aspect, the invention relates to methods and compositions for use in culturing Clostridium difficile and in producing C. difficile toxins.

Abstract: A method, container and kit for a two-component polymeric material, such as an epoxy coating includes filling a first pouch with a polymeric precursor material. A second pouch is filled with a curing agent. The first and second pouches are sealed. The first and second pouches are inserted into a mixing container and the mixing container is sealed. The first pouch can be filled with a first amount of polymeric precursor material. The second pouch can be filled with a second amount of curing agent with the first and second amounts totaling a first volume. The mixing container can have a second volume, with the first volume being greater than about half the second volume. The contents of the pouches can be transferred to the mixing container and mixed therein to react the components and dispense the resulting material.

Abstract: A method, container and kit for a two-component polymeric material, such as an epoxy coating includes filling a first pouch with a polymeric precursor material. A second pouch is filled with a curing agent. The first and second pouches are sealed. The first and second pouches are inserted into a mixing container and the mixing container is sealed. The first pouch can be filled with a first amount of polymeric precursor material. The second pouch can be filled with a second amount of curing agent with the first and second amounts totaling a first volume. The mixing container can have a second volume, with the first volume being greater than about half the second volume. The contents of the pouches can be transferred to the mixing container and mixed therein to react the components and dispense the resulting material.