Abstract: A device (10), for receiving fluid, having a block (12) which comprises: a chamber (14) comprising a top and a bottom; and a chamber outlet (20) at the bottom of the chamber (14). The block (12) further comprises a magnetic stirrer (30) suspended in the chamber (14), wherein the magnetic stirrer (30) terminates above the bottom of the chamber (14), and is rotatably supported inside the chamber (14). The block (12) further comprises a microchannel (26) fluidly connected to the chamber (14) via the chamber outlet (20). The microchannel (26) may be fluidly connected to a downstream reservoir (24), and an upstream reservoir (22). The device (10) is suited for mixing a bead/cell suspension and an oil-based fluid in the microchannel (26) such that they form an emulsion which comprises a plurality of droplets, wherein at least one of the droplets encapsulates a bead/cell.

Abstract: A device (10), for receiving fluid, having a block (12) which comprises: a chamber (14) comprising a top and a bottom; and a chamber outlet (20) at the bottom of the chamber (14). The block (12) further comprises a magnetic stirrer (30) suspended in the chamber (14), wherein the magnetic stirrer (30) terminates above the bottom of the chamber (14), and is rotatably supported inside the chamber (14). The block (12) further comprises a microchannel (26) fluidly connected to the chamber (14) via the chamber outlet (20). The microchannel (26) may be fluidly connected to a downstream reservoir (24), and an upstream reservoir (22). The device (10) is suited for mixing a bead/cell suspension and an oil-based fluid in the microchannel (26) such that they form an emulsion which comprises a plurality of droplets, wherein at least one of the droplets encapsulates a bead/cell.

Abstract: A reactor device (100) for reaction fluid comprising a reaction vessel (102) comprising: an end cap (104) comprising at least one passage (112) for the reaction fluid; and at least one tube (116) which extends through the reaction vessel (102). The reaction vessel is operable to receive a control fluid outside the at least one tube (116) for controlling the temperature inside the at least one tube (116). A manifold (200) is connectable to the end cap (104) and comprises at least one channel (206) for reaction fluid. An outlet (208) from the manifold (200) is in fluid communication with the tube (116). The end cap (104) has a thermal conductivity of greater than 1 watt per square meter kelvin to provide a thermal coupling between the control fluid and the manifold (200).

Abstract: A combined clamping and supporting ring for a reaction vessel having a radial flange at a junction between a vessel body and a vessel lid. The ring is capable of clamping around the flange to clamp the lid to the body and support the reaction vessel. The ring comprises a two-part collar comprising a U-shape main portion wide enough at one end to receive the flange of the reaction vessel in a direction parallel to the plane of the ring and a closure portion moveable with respect to the main portion to dose the collar. The collar has at least one support that fits under the flange of the reaction vessel to support the reaction vessel, and at least one clamping member that is arranged to engage above and below the flange to clamp the lid to the body when the collar is closed. At least two supports are provided in the main portion, one on each side of the U-shaped main portion.

Abstract: An apparatus (10) for creating a three dimensional device (100). The apparatus comprises a dispensing head (14) for dispensing material, and a base member (16) for receiving the material dispensed from the dispensing head (14). A controller (20) is provided for controlling the operation of the apparatus (10). The apparatus is operable to create the three dimensional device (100) by depositing a series of line deposits of material from the dispensing head (14) based on predetermined commands sent by the controller (20). The controller (20) is operable to control how the line deposits are dispensed to improve the sealing properties of the device (100).

Abstract: An apparatus and methods for monitoring the status of a cell that consumes oxygen. In one embodiment of the present invention, the method includes the steps of confining the cell in a sensing volume, measuring dynamically intracellular or extracellular signaling of the cell, and determining the status of the cell from the measured intracellular or extracellular signaling of the cell.

Abstract: An apparatus and methods for monitoring the status of a cell that consumes oxygen. In one embodiment of the present invention, the method includes the steps of confining the cell in a sensing volume, measuring dynamically intracellular or extracellular signaling of the cell, and determining the status of the cell from the measured intracellular or extracellular signaling of the cell.

Abstract: An apparatus (10) for performing microfluidic processes comprising a base (50), a plurality of fluidic modules (20) releasably attached to the base (50), each fluidic module (20) comprising a fluid port (25) and a microfluidic manifold module (40) comprising a plurality of ports (45). A frame (70) is attached to the base (50) for releasably retaining the microfluidic manifold module (40), the frame (70) being moveable relatively to the base (50) to move the microfluidic manifold module (40) into contact with the fluidic modules (20) such that each fluid port (25) of the fluidic modules (20) aligns and seals with a respective port (45) on the microfluidic manifold module (40) thus completing a microfluidic circuit. A method for constructing and testing the apparatus (10) is also disclosed.