Abstract: A unique aerosol-compatible cell culture exposure system is described that allow serial dilution and delivery of aerosols, reactive gases, and VOCs at air-liquid interface (ALI) under physiologically relevant conditions. Generally, the cell culture system comprise one or more dilution manifolds, one or more flow splitters, and a sealed exposure chamber that houses a commercially-available cell culture plate with corresponding transwells.

Abstract: Methods and systems are provided for processing PFAS-contaminated waste via thermomechanochemical (TMC) processing. In one example, a system may include a TMC processing chamber including a milling system and coupled to a heating system, the heating system configured to co-operate with the milling system to process the waste material. A plurality of containers including the waste material in one or more of a liquid-solid state, a semi-wet or slurry solid state, and a dry solid state are coupled to the TMC chamber.

Abstract: An vitro air-liquid interface (ALI) exposure of cultured cells makes it possible to examine the toxicological properties of the tested air. A method for evaluating effect of a polluting air stream comprises the steps of exposing cells to a membrane until adhesion of the cells has occurred, feeding cells periodically until there is a confluent monolayer of cells on said membranes, aspirating off non-adherent cells, applying fresh media, exposing the cells to over-head stream containing pollutants, removing the cells from the membranes that have been exposed to the over-head stream, then measuring the cells' response to toxins in the over-head stream.