Abstract: The present disclosure relates to particle-based methods and kits for extracting nucleic acids, such as with magnetic or magnetically-responsive particles, from a wide variety of biological samples. The methods and kits of this disclosure may be used to extract nucleic acids with high purity and recovery (compared to conventional methods, such as using spin-columns) from simple through complex biological samples. The methods and kits of this disclosure may be used in high-throughput extractions or may be amenable to use in high-throughput applications.