Abstract: Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased KM for NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.

Abstract: There is disclosed a first stub short bonded across conductive runners of a tuning stub at a distance preferably greater than a distance resulting in the desired frequency of operation of a voltage controlled oscillator tuned by the tuning stub. Thereafter, the voltage controlled oscillator is powered and tested to determine the frequency of operation of the voltage controlled oscillator. The position of a second stub short is determined based on the frequency of oscillation of the oscillator due to the presence of the first stub short, the geometry of the tuning stub and the desired frequency of operation of the voltage controlled oscillator. A second stub short is precisely positioned along the stub using automated equipment, relative to the position of the first stub short, to result in the desired frequency of operation of the voltage controlled oscillator.