Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.

Abstract: An orifice reducer with crumple zones well suited for use with an extrusion blow molded bottle having an uneven surface finish. The device includes a circumferential wall having a number of vertically extending reduced thickness regions, preferably spaced symmetrically about the circumferential wall. The reduced thickness regions deform upon insertion of the orifice reducer into the neck of a bottle to account for irregularity of the surface finish of the bottle. This allows the orifice reducer to be more easily inserted into standard extrusion blow molded bottles.