Abstract: The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences which encode these proteins. More specifically, in preferred embodiments, pesticidal proteins of approximately 40-50 kDa and of approximately 10-15 kDa are used for controlling corn rootworms. Also described are novel pesticidal isolates of Bacillus thuringiensis.

Abstract: The subject invention concerns new classes of insecticidal proteins obtainable from Bacillus thuringiensis, and polynucleotides that encode these proteins. The subject invention also includes transgenic cells and plants that produce these proteins. The proteins are preferably in the 10-15 kDa and 40-50 kDa size range.

Abstract: The subject invention pertains to B.t. toxins active against pests. More specifically, the subject invention pertains to truncated Cry6A toxins. These activated toxins are particularly effective for controlling coleopteran pests such as the corn rootworm and the alfalfa weevil.

Abstract: The present invention provides polynucleotides that a protein solubility responsive promoter operatively liked to a reporter gene and a genetic reporter system comprising these polynucleotides together with an expression construct for a target protein. The invention also provides cells comprising polynucleotides of the invention and the genetic reporter system. These compositions are useful to monitor the solubility of a target protein in a cell and to identify mutations to the cell or mutations to a polynucleotide encoding the target protein that alters the solubility of the target protein. The invention further provides method to identify variations in a protein biosynthetic process that alter the solubility of a target protein and methods to screen an expression library of recombinant clones to identify clones that express soluble proteins. Finally, the invention discloses a novel method of identifying an antibiotic agent.

Abstract: The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences that encode these proteins. In preferred embodiments, these pesticidal protein have molecular weights of approximately 40-50 kDa and of approximately 10-15 kDa.

Abstract: The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences that encode these proteins. In preferred embodiments, these pesticidal proteins have molecular weights of approximately 40-50 kDa and of approximately 10-15 kDa.

Abstract: The subject invention concerns new classes of pesticidal toxins and the polynucleotide sequences which encoded these toxins. Also described are novel pesticidal isolates of Bacillus thuringiensis.

Abstract: The subject invention concerns new classes of insecticidal proteins obtainable from Bacillus thuringiensis, and polynucleotides that encode these proteins. The subject invention also includes transgenic cells and plants that produce these proteins. The proteins are preferably in the 10-15 kDa and 40-50 kDa size range.

Abstract: The present invention provides polynucleotides that a protein solubility responsive promoter operatively liked to a reporter gene and a genetic reporter system comprising these polynucleotides together with an expression construct for a target protein. The invention also provides cells comprising polynucleotides of the invention and the genetic reporter system. These compositions are useful to monitor the solubility of a target protein in a cell and to identify mutations to the cell or mutations to a polynucleotide encoding the target protein that alters the solubility of the target protein. The invention further provides method to identify variations in a protein biosynthetic process that alter the solubility of a target protein and methods to screen an expression library of recombinant clones to identify clones that express soluble proteins. Finally, the invention discloses a novel method of identifying an antibiotic agent.

Abstract: The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences which encode these proteins. More specifically, in preferred embodiments, pesticidal proteins of approximately 40-50 kDa and of approximately 10-15 kDa are used for controlling corn rootworms. Also described are novel pesticidal isolates of Bacillus thuringiensis.

Abstract: The present invention relates to the production of a non-transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The present invention also relates to the use of a recombinagenic oligonucleobase to make a desired mutation in the chromosomal or episomal sequences of a plant in the gene encoding for 5-enol pyruvylshikimate-3-phosphate synthase (EPSPS). The mutated protein, which substantially maintains the catalytic activity of the wild-type protein, allows for increased resistance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells as compared to the wild-type plant irrespective of the presence or absence of the herbicide.

Abstract: The subject invention concerns new classes of pesticidal toxins and the polynucleotide sequences which encode these toxins. Also described are novel pesticidal isolates of Bacillus thuringiensis.

Abstract: The subject invention pertains to B.t. toxins active against pests. More specifically, the subject invention pertains to truncated Cry6A toxins. These activated toxins are particularly effective for controlling coleopteran pests such as the corn rootworm and the alfalfa weevil.

Abstract: The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences which encode these proteins. More specifically, in preferred embodiments, pesticidal proteins of approximately 40-50 kDa and of approximately 10-15 kDa are used for controlling corn rootworms. Also described are novel pesticidal isolates of Bacillus thuringiensis.

Abstract: The subject invention pertains to B.t. toxins active against pests. More specifically, the subject invention pertains to truncated Cry6A toxins. These activated toxins are particularly effective for controlling coleopteran pests such as the corn rootworm and the alfalfa weevil.

Abstract: The subject invention concerns new classes of pesticidal toxins and the polynucleotide sequences which encode these toxins. Also described are novel pesticidal isolates of Bacillus thuringiensis.

Abstract: The subject invention concerns new classes of pesticidal toxins and the polynucleotide sequences which encode these toxins. Also described are novel pesticidal isolates of Bacillus thuringiensis.

Abstract: The subject invention pertains to B.t. toxins active against pests. More specifically, the subject invention pertains to truncated Cry6A toxins. These activated toxins are particularly effective for controlling coleopteran pests such as the corn rootworm and the alfalfa weevil.

Abstract: The activity of pesticides is enhanced by application of a zwittermicin antibiotic in combination with insecticides. In particular, zwittermicin enhances the activity of Bacillus thuringiensis .delta.-endotoxins.