Abstract: Provided herein is a biological detection system and method of use wherein the biological detection system comprises at least one mixer or liquid bridge for combining at least two liquid droplets and an error correction system for detecting whether or not proper mixing or combining of the two component droplets have occurred.

Abstract: A liquid handling system of a PCR system is instructed to obtain a matrix of samples and reagents for a PCR experiment. A fluid pumping system of the PCR system is instructed to maintain a continuous flow of a transport fluid through a plurality of micro-channels that allows the mixture of the samples and the reagents producing a plurality of mixed sample droplets. One or more post-bridge detection values are received from a post-bridge detection system of the PCR system for each mixed sample droplet to determine if the mixed sample droplet is mixed correctly. A thermocycler of the PCR system is instructed to maintain one or more temperatures for cycling the temperature of the plurality of mixed sample droplets. One or more endpoint detection values are received from an endpoint detection system of the PCR system for each mixed sample droplet to analyze the PCR experiment.

Abstract: Provided herein is a biological detection system and method of use wherein the biological detection system comprises at least one mixer or liquid bridge for combining at least two liquid droplets and an error correction system for detecting whether or not proper mixing or combining of the two component droplets have occurred.

Abstract: A liquid handling system of a PCR system is instructed to obtain a matrix of samples and reagents for a PCR experiment. A fluid pumping system of the PCR system is instructed to maintain a continuous flow of a transport fluid through a plurality of micro-channels that allows the mixture of the samples and the reagents producing a plurality of mixed sample droplets. One or more post-bridge detection values are received from a post-bridge detection system of the PCR system for each mixed sample droplet to determine if the mixed sample droplet is mixed correctly. A thermocycler of the PCR system is instructed to maintain one or more temperatures for cycling the temperature of the plurality of mixed sample droplets. One or more endpoint detection values are received from an endpoint detection system of the PCR system for each mixed sample droplet to analyze the PCR experiment.

Abstract: Provided herein is a biological detection system and method of use wherein the biological detection system comprises at least one mixer or liquid bridge for combining at least two liquid droplets and an error correction system for detecting whether or not proper mixing or combining of the two component droplets have occurred.