Abstract: A new latch device for a screen door includes inside and outside handle assemblies. Each assembly has a lever adapted to be pivotably connected, respectively, to the inboard and outboard side of the screen door. The inboard lever has a paddle at one end and a stem at the other. The stem extends through an opening formed in the screen door. When the paddle is depressed, the stem moves along a cam surface formed in the outboard lever. This causes the outboard lever to disengage the fastener on the solid door to disconnect the solid door from the screen door. Additionally, when the screen door is in its latched position and not connected to the solid door, depressing the paddle causes the latching end of the outboard lever to disengage the doorjamb so that the screen door can be moved independently from its latched position into its open position.

Abstract: A handle assembly is provided for a dual main door and screen door assembly of an RV or trailer. The assembly includes a main door module and a screen door module. The main door module includes an outside handle which retracts the main door plunger when the doors are both closed. The screen door module includes an inside handle which retracts the main door plunger from inside the RV or trailer when both doors are closed. The inside handle also retracts the screen door plunger when from inside the RV or trailer when the screen door is closed and the main door is open. An outside handle on the screen door module retracts the screen door plunger from the outside when the main door is open and the screen door is closed.

Abstract: A clamp frame is disclosed for securing a window frame in a building structure opening by hand without using fasteners. The clamp frame comprises a unitary extruded plastic member that defines a cross section having an integrally formed connection element that mates with a complementarily shaped portion of a window frame to connect the clamp frame and the window frame without fasteners along the length of the clamp frame. The cross section includes a clamp element having at least a leg and a lever. The leg and lever are each connected to the connection element by a living hinge and are movable relative to the connection element between an open position and an overlapping locked position, which results in a clamp load holding together the clamp frame and window frame without the use of fasteners. One end of the leg of the clamp element is constantly urged against the building structure by the lever when the clamp element is in the locked position.

Abstract: A plastic window frame assembly is disclosed that includes rigid molded corner sections. Each of the corner sections has opposite ends. A pair of offset tabs extend outward from each of the opposite ends. The corners are molded so as to include integrally formed non-rigid seals. Lineal rail sections are located between the corner sections. Each of the rail sections has channels, one each formed in an exterior and an interior surface of the rail section for receiving, respectively, the offset tabs of the corner sections to locate and to align the corner sections with a rail section. The channels include a glazing channel extending along the length of the rail section for receiving a glass or plastic pane. The tabs are connected to a rail section such that the tabs and a portion of a rail section together define stake holds, each of which proceeds from an exterior surface of the frame and extends therethrough but does not penetrate the interior surface or the tabs of the frame.

Abstract: A clamp frame is disclosed for securing a window frame in a building structure opening by hand without using fasteners. The clamp frame comprises a unitary extruded plastic member that defines a cross section having an integrally formed connection element that mates with a complementarily shaped portion of a window frame to connect the clamp frame and the window frame without fasteners along the length of the clamp frame. The cross section includes a clamp element having at least a leg and a lever. The leg and lever are each connected to the connection element by a living hinge and are movable relative to the connection element between an open position and an overlapping locked position, which results in a clamp load holding together the clamp frame and window frame without the use of fasteners. One end of the leg of the clamp element is constantly urged against the building structure by the lever when the clamp element is in the locked position.

Abstract: A multi-pane flush closing window assembly for a vehicle that has a body enclosing a passenger compartment is secured in an opening in the vehicle body by a clamp ring fastened to the interior wall of the vehicle body. The improved window assembly includes at least one fixed sub-assembly and at least one sliding sub-assembly supported by a frame. The fixed sub-assembly has a sash, and removable fasteners extend through the peripheral edge of the sash into the interior side of the frame so that the fixed sub-assembly can be independently disassembled from the interior of the vehicle without removing the frame or the clamp ring. The sliding sub-assembly is removably secured to the frame by an improved multi-piece guide track and slide pin assembly that allows removal of the sliding sub-assembly without removing the frame. The slide pins are removably secured to opposite sides of the sliding sub-assembly.

Abstract: A plastic window frame assembly is disclosed that includes rigid molded corner sections. Each of the corner sections has opposite ends. A pair of offset tabs extend outward from each of the opposite ends. The corners are molded so as to include integrally formed non-rigid seals. Lineal rail sections are located between the corner sections. Each of the rail sections has channels, one each formed in an exterior and an interior surface of the rail section for receiving, respectively, the offset tabs of the corner sections to locate and to align the corner sections with a rail section. The channels include a glazing channel extending along the length of the rail section for receiving a glass or plastic pane.

Abstract: Herein-described are various methods for making a vaccine that are made of re-assembled virus like particles (VLP). First, the VLPs are disassembled into encapsidation intermediate populations. Each encapsidation intermediate population undergoes, for instance, chemical conjugation of unique peptide or nucleic moieties to form separate populations. Thereafter, a predetermined amount of each of the several (one or more) different encapsidation intermediates from the different populations is mixed and joined, forming intact VLPs, surrounding a nucleic acid core, that are composed of different encapsidation intermediate such that the reassembled VLP displays more than one peptide or nucleic acid. The nucleic acid can function either as a scaffold alone or can be engineered for the expression of an immunomodulatory protein in a eukaryotic cell.

Abstract: Compositions for treating papillomavirus (PV) infection in a subject include a PV L2 polypeptide produced from a eukaryotic expression system.

Abstract: A multi-pane flush closing window assembly for a vehicle includes any one of a combination of fixed sub-assemblies and/or sliding sub-assemblies supported by a frame. The sub-assemblies may be conveniently assembled and disassembled from the frame without removing the frame or window securing structure from the vehicle. Man hours, tooling, and costs associated with flush window assembly and repairs are reduced as a result. The fixed sub-assembly has a sash, and removable fasteners extend through the peripheral edge of the sash into the interior side of the frame. The sliding sub-assembly is removably secured to the frame by an improved multi-piece guide track and slide pin assembly. The slide pins are removably secured to opposite sides of the sliding sub-assembly. A lock nut or coil spring provides a means for adjusting the slide pin so that the sliding sub-assembly assumes an optimal flush fit with the frame and the fixed sub-assembly.

Abstract: The invention provides a fusion protein comprising a plant virus coat protein and a GDF8 peptide domain, or antigenic fragment of the GDF8 peptide domain. Plant virus vectors expressing the fusion protein and methods of using these vectors are also provided.

Abstract: Display of peptides or proteins in an ordered, repetitive array, such as on the surface of a virus-like particle, is known to induce an enhanced immune response relative to vaccination with the “free” protein antigen. The 2100 coat proteins comprising the rod-shaped capsid of Tobacco mosaic virus (TMV) can accommodate short peptide insertions into the primary sequence, but the display of larger protein moieties on the virion surface by genetic fusions to the capsid protein has not been possible. Since TMV lacks surface exposed residues compatible with commonly available linker chemistries, we employed a randomized library approach to introduce a reactive lysine at the externally located at the amino-terminus of the coat protein. We found that we could easily control the extent of virion conjugation and demonstrated stoichiometric biotinylation of the introduced lysine.

Abstract: The invention provides a fusion protein comprising a plant virus coat protein and a GDF8 peptide domain, or antigenic fragment of the GDF8 peptide domain. Plant virus vectors expressing the fusion protein and methods of using these vectors are also provided.

Abstract: Juvenile-onset recurrent respiratory papillomatosis is treated using active vaccination or passive immune therapy of neutralizing antibodies against HPV L2 neutralizing epitopes.

Abstract: Compositions for treating papillomavirus (PV) infection in a subject include a PV L2 polypeptide produced from a eukaryotic expression system.

Abstract: We describe here restriction endonucleases and their uses. Restriction endonucleases are useful in finding single nucleotide polymorphisms. They are also useful in an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences.

Abstract: A plurality of proteins of interest, or peptides of interest, or other genetically expressed materials, are screened and subsequently produced using any of a variety of expression systems. The plurality of proteins are extracted from a plurality of separate, processed green juices, each green juice containing one of the proteins of interest. A multi-channel apparatus processes the various green juices, one green juice per channel. The apparatus is computer controlled such that the various valves in each channel and pump are controlled in an automated manner to extract each protein of interest and deliver each protein of interest into its own storage vessel.

Abstract: We describe here an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences, by making a heteroduplex polynucleotide from two non-identical polynucleotides; introducing a nick in one strand at or near a base pair mismatch site; removing mismatched base(s) from the mismatch site where the nick occurred; and using the opposite strand as template to replace the removed base(s) with bases that complement base(s) in the first strand. By this method, information is transferred from one strand to the other at sites of mismatch.

Abstract: The present invention relates to a composition, which includes a hepatitis B surface antigen stabilized with a milk protein and/or a milk protein component. This composition can be used in an oral vaccine for treatment of hepatitis B. The present invention further relates to methods of immunizing a subject against hepatitis, methods of administrating the composition of the present invention, and methods of producing a stabilized hepatitis B surface antigen protein.