Patents by Inventor Mark Lim

Mark Lim has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Compositions and methods for immunoaffinity purification
    Patent number: 7534861
    Abstract: A hybrid polypeptide composed of a p53 epitope peptide and a desired functional protein are produced by recombinant DNA techniques. A DNA expression vector is constructed that includes segments of DNA coding for the epitope peptide and the desired functional protein. An optional linking portion is contemplated. The linking portion of the epitope peptide is cleavable at a specific amino acid residue adjacent the functional protein by use of a sequence specific proteolytic enzyme or chemical proteolytic agent. The hybrid polypeptide expressed by the host cells transformed by the cloning vector is removed therefrom and purified by affinity chromatography techniques by use of an immobilized antibody specific to the antigenic portion of the epitope peptide. The protein is then cleaved from the isolated hybrid polypeptide with an appropriate proteolic enzyme or chemical agent, thereby releasing the mature functional protein in highly purified, highly active state.
    Type: Grant
    Filed: January 10, 2003
    Date of Patent: May 19, 2009
    Assignee: Ambergen, Inc.
    Inventors: Kenneth J. Rothschild, Sadanand Gite, Jerzy Olejnik, Mark Lim
  • Methods for the detection, analysis and isolation of nascent proteins
    Patent number: 7312060
    Abstract: This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride(4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)dyes.
    Type: Grant
    Filed: January 10, 2003
    Date of Patent: December 25, 2007
    Assignee: Ambergen, Inc.
    Inventors: Kenneth J. Rothschild, Sadanand Gite, Jerzy Olejnik, Mark Lim
  • Methods for the detection, analysis and isolation of nascent proteins
    Patent number: 7252932
    Abstract: This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) dyes.
    Type: Grant
    Filed: August 23, 2000
    Date of Patent: August 7, 2007
    Assignee: AmberGen, Inc.
    Inventors: Kenneth J. Rothschild, Sadanand Gite, Jerzy Olejnik, Sergey Mamaev, Mark Lim
  • Compositions and methods for immunoaffinity purification
    Publication number: 20040138413
    Abstract: A hybrid polypeptide composed of a p53 epitope peptide and a desired functional protein are produced by recombinant DNA techniques. A DNA expression vector is constructed that includes segments of DNA coding for the epitope peptide and the desired functional protein. An optional linking portion is contemplated. The linking portion of the epitope peptide is cleavable at a specific amino acid residue adjacent the functional protein by use of a sequence specific proteolytic enzyme or chemical proteolytic agent. The hybrid polypeptide expressed by the host cells transformed by the cloning vector is removed therefrom and purified by affinity chromatography techniques by use of an immobilized antibody specific to the antigenic portion of the epitope peptide. The protein is then cleaved from the isolated hybrid polypeptide with an appropriate proteolic enzyme or chemical agent, thereby releasing the mature functional protein in highly purified, highly active state.
    Type: Application
    Filed: January 10, 2003
    Publication date: July 15, 2004
    Applicant: AmberGen, Inc.
    Inventors: Kenneth J. Rothschild, Sadanand Gite, Jerzy Olejnik, Mark Lim
  • Methods for the detection, analysis and isolation of nascent proteins
    Publication number: 20040014071
    Abstract: This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) dyes.
    Type: Application
    Filed: January 10, 2003
    Publication date: January 22, 2004
    Applicant: AmberGen, Inc.
    Inventors: Kenneth J. Rothschild, Sadanand Gite, Jerzy Olejnik, Mark Lim