Abstract: A method for excluding an aggressive incompatibility (AI) trait from Agaricus bisporus mushroom strains, wherein the method includes mating a culture of a hybrid mushroom line designated B18287-s82, a representative culture of the line having been deposited under NRRL Accession No. 68168, with a culture of the white mushroom line designated WBL-s290, to obtain an F1 hybrid mushroom strain designated J19109, a representative culture of the F1 strain having been deposited under NRRL Accession No. 68163. Upon fruiting a culture of the new F1 strain designated J19109 to obtain homokaryotic spores therefrom, one obtains cultures of homokaryotic lines from the homokaryotic spores from F1 strain J19109 and selects a culture of a homokaryotic line from the F1 strain J19109. The culture of a homokaryotic line from F1 strain J19109 is mated with a culture of the mushroom line designated J11500-s80, to obtain an F2 hybrid mushroom strain.

Abstract: The present invention relates to the development of a homokaryotic Agaricus bisporus (Lange) Imbach mushroom fungus line culture designated N-s34 and to cultures obtained, descended, or otherwise derived from line N-s34. More particularly, the present invention relates to cultures incorporating at least one set of chromosomes having a genotype present in the genotype of the chromosomes found in line N-s34. The present invention further relates to F1 hybrids, and to a particular F1 hybrid strain, designated LA3782, descended from N-s34. This particular strain indeed displays an excellent yield weight of the harvested crop, especially in the third-flush, and a very good shelf-life of the mushroom products. The invention additionally relates to progeny, lines and strains derived from or descended from, or otherwise developed or obtained from, line N-s34 and from said hybrid strain LA3782. The invention further relates to methods of use of the cultures described hereinabove.