Abstract: An assay plate system for assaying a cell culture model, such as an organoid, comprises an assay plate, an upper surface, a grid array of wells comprising at least two rows of wells, each row comprising at least two wells, each well having an open top formed in the upper surface and a closed transparent base, and at least two columns of wells, each column comprising at least three wells, each well having an open top formed in the upper surface and a closed transparent base. A first gasket comprising a plate having a top surface and a bottom surface comprising a plurality of elongated recesses is provided that together with the upper surface of the assay plate define a fluidic conduit for each row of wells providing fluidic communication between the first fluid inlet aperture, the row of wells and the first fluid outlet aperture in series.

Abstract: A beverage apparatus, hand-holdable by a user of the beverage apparatus to be portable, can include a chamber housing that includes a chamber for storing a consumable liquid. The beverage apparatus can include a dispensing assembly. The dispensing assembly can retain a vessel or pod. The dispensing assembly can be operatively controllable by a controller to output the additive from the vessel into the consumable liquid. The beverage apparatus can include a receptacle member that is provided in the shape of a volcano or cylinder. The receptacle member can serve to house a pod. A skirt structure can be mounted to a lower portion of the beverage apparatus. The skirt can be flexible so as to accommodate for reciprocal movement of the pod, in a dispense event. Related features are disclosed.

Abstract: A beverage apparatus, hand-holdable by a user of the beverage apparatus to be portable, can include a chamber housing that includes a chamber for storing a consumable liquid. The beverage apparatus can include a dispensing assembly. The dispensing assembly can retain a vessel or pod. The dispensing assembly can be operatively controllable by a controller to output the additive from the vessel into the consumable liquid. The beverage apparatus can include a receptacle member that is provided in the shape of a volcano or cylinder. The receptacle member can serve to house a pod. A skirt structure can be mounted to a lower portion of the beverage apparatus. The skirt can be flexible so as to accommodate for reciprocal movement of the pod, in a dispense event. Related features are disclosed.

Abstract: A beverage apparatus hand-holdable by a user to be portable, can include a beverage chamber housing that includes a chamber for storing a consumable liquid. The beverage apparatus can include a dispensing assembly that includes a receptacle. The receptacle can retain a vessel. The vessel can include an electronic tag and can contain an additive. The dispensing assembly can be operatively controllable by a controller to output the additive from the vessel into the consumable liquid. The beverage apparatus can include one or more sensors, devices, or assemblies that can be used to detect a volume of liquid in the chamber or a liquid level in the chamber. The beverage apparatus can include an apparatus computer processor portion (ACP) and an apparatus database portion.

Abstract: Systems and methods are disclosed to mix a base ingredient, such as water, with an additive, such as a cleaning concentrate. A dispensing system of the disclosure can include a container filling apparatus. The container filling apparatus can include one or more source containers that contain an additive to be mixed with water or other base ingredient. The container filling apparatus can include a vacuum manifold and a Venturi body assembly (VBA). The additive can be flowed through the vacuum manifold and into the VBA. In the VBA, the additive can be mixed with water or other base ingredient, and output to a receiving container. As the additive is flowed through the vacuum manifold, vacuum pressure in the pressure manifold can be measured by a pressure sensor. A controller can input pressure data from the pressure sensor over the course of a dispense event. Over the course of a dispense event, flow of additive can be varied or modulated based on the pressure data.

Abstract: Systems and methods are disclosed to mix a base ingredient, such as water, with an additive, such as a cleaning concentrate. A dispensing system of the disclosure can include a container filling apparatus. The container filling apparatus can include one or more source containers that contain an additive to be mixed with water or other base ingredient. The container filling apparatus can include a vacuum manifold and a Venturi body assembly (VBA). The additive can be flowed through the vacuum manifold and into the VBA. In the VBA, the additive can be mixed with water or other base ingredient, and output to a receiving container. As the additive is flowed through the vacuum manifold, vacuum pressure in the pressure manifold can be measured by a pressure sensor. A controller can input pressure data from the pressure sensor over the course of a dispense event. Over the course of a dispense event, flow of additive can be varied or modulated based on the pressure data.

Abstract: A beverage apparatus hand-holdable by a user to be portable, can include a beverage chamber housing that includes a chamber for storing a consumable liquid. The beverage apparatus can include a dispensing assembly that includes a receptacle. The receptacle can retain a vessel. The vessel can include an electronic tag and can contain an additive. The dispensing assembly can be operatively controllable by a controller to output the additive from the vessel into the consumable liquid. The beverage apparatus can include one or more sensors, devices, or assemblies that can be used to detect a volume of liquid in the chamber or a liquid level in the chamber. The beverage apparatus can include an apparatus computer processor portion (ACP) and an apparatus database portion.

Abstract: The systems and methods provide a container assembly comprising: a container having a known storage capacity for storing a liquid; an additive dispensing assembly, the additive dispensing assembly dispensing variable, non-zero quantities of one or more additives into the liquid stored in the container; one or more vessels that each contain one of the additives, of the one or more additives, to be dispensed into the liquid; and a gas dispensing assembly, the gas dispensing assembly releasing a gas into the liquid stored in the container, and the gas dispensing assembly including: an onboard gas tank; a valve assembly; and a gas outlet, and the valve assembly controlling flow of gas from the onboard gas tank, through the valve assembly, and to the gas outlet so as to output the gas into the liquid; and wherein the valve assembly, to perform the controlling the flow of gas, is movable between: an open position, in which flow of gas is allowed to flow from the onboard gas tank to the gas outlet; and a closed posi

Abstract: The systems and methods provide a container assembly comprising: a container having a known storage capacity for storing a liquid; an additive dispensing assembly, the additive dispensing assembly dispensing variable, non-zero quantities of one or more additives into the liquid stored in the container; one or more vessels that each contain one of the additives, of the one or more additives, to be dispensed into the liquid; and a gas dispensing assembly, the gas dispensing assembly releasing a gas into the liquid stored in the container, and the gas dispensing assembly including: an onboard gas tank; a valve assembly; and a gas outlet, and the valve assembly controlling flow of gas from the onboard gas tank, through the valve assembly, and to the gas outlet so as to output the gas into the liquid; and wherein the valve assembly, to perform the controlling the flow of gas, is movable between: an open position, in which flow of gas is allowed to flow from the onboard gas tank to the gas outlet; and a closed posi

Abstract: The disclosure provides bottle apparatuses, pods or other storage containers to be used in bottle apparatuses, beverage dispensing apparatuses, and methods of making and using the same in accordance with principles of the disclosed subject matter. A bottle apparatus in accordance with the disclosure can include a neck portion; a bottle portion having an upper end and a lower end, the bottle portion defining an internal volume, and the neck portion attached to the upper end of the bottle portion; a base portion attached to the lower end of the bottle portion; a dispense assembly attached to the neck portion, and the dispense assembly providing for outflow of consumable liquid from the bottle portion; and a flow meter assembly disposed within the base portion, and the flow meter assembly monitoring an amount of the consumable liquid that flows out of the dispense assembly.

Abstract: A portable, self-contained beverage apparatus includes a container assembly having a known storage capacity for storing a consumable liquid, and a dispensing assembly disposed within the container assembly that dispenses variable, non-zero quantities of additives into the consumable liquid. The dispensing assembly includes multiple apertures structured and arranged to retain vessels containing the additives to be dispensed into the consumable liquid. The beverage apparatus also includes a level sensor disposed within the container assembly that determines a consumable liquid level of the consumable liquid stored in the container assembly. In certain embodiments, one or more positive displacement pumping mechanisms are configured to pump additive liquid from additive containers into a beverage chamber. Other features relate to audio engagement processing. Other features relate to situational processing. Other features relate to group engagement processing.

Abstract: The systems and methods provide a container assembly comprising: a container having a known storage capacity for storing a liquid; an additive dispensing assembly, the additive dispensing assembly dispensing variable, non-zero quantities of one or more additives into the liquid stored in the container; one or more vessels that each contain one of the additives, of the one or more additives, to be dispensed into the liquid; and a gas dispensing assembly, the gas dispensing assembly releasing a gas into the liquid stored in the container, and the gas dispensing assembly including: an onboard gas tank; a valve assembly; and a gas outlet, and the valve assembly controlling flow of gas from the onboard gas tank, through the valve assembly, and to the gas outlet so as to output the gas into the liquid; and wherein the valve assembly, to perform the controlling the flow of gas, is movable between: an open position, in which flow of gas is allowed to flow from the onboard gas tank to the gas outlet; and a closed posi

Abstract: A computer-readable recording medium including a program which is executed by a computer apparatus to provide a video game comprising a virtual game world presented to a player of the video game, and a plurality of characters in the virtual game world including a first character, and a second character controlled by the player, the program causing the computer apparatus to function as: a state indicator generating unit configured to generate a state indication, wherein the state indication is an indication of an emotional or physical state of the first character; and a coupling indicator generating unit configured to generate a coupling indication, wherein the coupling indication is an indication of a coupling occurring between the emotional or physical state of the first character and an emotional or physical state of the second character.

Abstract: Bottle apparatuses, storage containers used in bottle apparatuses, beverage dispensing apparatuses, and methods of making and using the same are disclosed. A bottle apparatus can include a neck portion; a bottle portion having an upper end and a lower end, the bottle portion defining an internal volume, and the neck portion attached to the upper end of the bottle portion; a base portion attached to the lower end of the bottle portion; a dispense assembly attached to the neck portion, and the dispense assembly providing for outflow of consumable liquid from the bottle portion; and a flow meter assembly disposed within the base portion, and the flow meter assembly monitoring an amount of the consumable liquid that flows out of the dispense assembly.

Abstract: A portable, self-contained beverage apparatus includes a container assembly having a known storage capacity for storing a consumable liquid, and a dispensing assembly disposed within the container assembly that dispenses variable, non-zero quantities of additives into the consumable liquid. The dispensing assembly includes multiple apertures structured and arranged to retain vessels containing the additives to be dispensed into the consumable liquid. The beverage apparatus also includes a level sensor disposed within the container assembly that determines a consumable liquid level of the consumable liquid stored in the container assembly. In certain embodiments, one or more positive displacement pumping mechanisms are configured to pump additive liquid from additive containers into a beverage chamber.

Abstract: A portable, self-contained beverage apparatus includes a container assembly having a known storage capacity for storing a consumable liquid, and a dispensing assembly disposed within the container assembly that dispenses variable, non-zero quantities of additives into the consumable liquid. The dispensing assembly includes multiple apertures structured and arranged to retain vessels containing the additives to be dispensed into the consumable liquid. The beverage apparatus also includes a level sensor disposed within the container assembly that determines a consumable liquid level of the consumable liquid stored in the container assembly. In certain embodiments, one or more positive displacement pumping mechanisms are configured to pump additive liquid from additive containers into a beverage chamber. Other features relate to audio engagement processing. Other features relate to situational processing. Other features relate to group engagement processing.

Abstract: The present disclosure relates to methods for inactivating a Zika virus which can be used in vaccines and immunogenic compositions. The present disclosure also relates to a method for determining the completeness of inactivation of an arbovirus preparation.

Abstract: The present disclosure relates to Zika virus vaccines and immunogenic compositions having one or more antigens from a Zika virus (e.g., a Zika virus clonal isolate, a non-human cell adapted Zika virus, etc.), and methods of treatments and uses thereof.