Abstract: A device, system and method for treating biological cells includes a voltage source, a half-controlled bridge connected to the voltage source, and a load connected across the half-controlled bridge. The half-controlled bridge includes a first switch, a second switch, a first diode and a second diode. The load includes an inductor connected in parallel with a cell or chamber. A controller is connected to the first and second switches and operates the first switch and the second switch to selectively generate one or more bipolar pulses, wherein each bipolar pulse comprises a positive polarity voltage pulse and a negative polarity voltage pulse with a negligible delay between the positive polarity voltage pulse and the negative polarity voltage pulse.

Abstract: A high-voltage bipolar rectangular pulse generator using a high efficiency solid-state boosting front-end and an H-bridge output stage is described. The topology of the circuit generates rectangular pulses with fast rise time and allows easy step-up input voltage. In addition, the circuit is able to adjust positive or negative pulse width, dead-time between two pulses, and operating frequency. The intended application for such circuit is algae cell membrane rupture for oil extraction, although additional applications include biotechnology and plasma sciences medicine, and food industry.