Abstract: A method and apparatus for fabricating a multi-layer optical article includes grasping an outer surface of a first substrate with a first holder, whereby the outer surface of the first substrate is held to an inner surface of the first holder, grasping an outer surface of a second substrate with a second holder, whereby the outer surface of the second substrate is held to an inner surface of the second holder, arranging the inner surfaces of the holders to face one another, arranging at least one spacer element between the holders, disposing an adherent on the inner surface of one of the substrates, moving the holders together such that the holders contact the at least one spacer and the substrates contact the adherent, and at least partially curing the adherent while the inner surfaces of the holders are in a selected angular relationship.

Abstract: A novel photoimageable system for a two-chemistry system containing liquid photoreactive asymmetric acrylate compound containing sulfur, aromatic moieties, and optionally bromine, and an aluminum salt compound is disclosed. The photoimageable system has high dynamic range (M/#) and sensitivity and unexpectedly high temperature and high humidity resistance. The photoimageable system retains its dynamic range when exposed to 60° C. for 4 weeks while a composition without the aluminum salt compound lost 75% of its dynamic range under similar exposure conditions. In one embodiment, 2-10 weight % of a thio-butylacrylate dissolved in a two-component urethane matrix containing 0.002 to 1 weight % of the aluminum salt compound showed a dynamic range of greater than 5 for a 200 microns thick sample and retained more than 80% of the dynamic range after 4 weeks exposure at 60° C.

Abstract: The present invention relates to mutants of the green fluorescent protein having improved fluorescent properties at 37.degree. C. The mutants provide for improved methods of monitoring gene expression, e.g., for use as cell markers or protein expression indicators in prokaryotic and, especially, eucaryotic systems where the standard physiological temperature is 37.degree. C.