Patents by Inventor Mark N. Bobrow
Mark N. Bobrow has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230313297Abstract: Compositions, kits and methods for detecting a plurality of targets are provided herein. A probe-set composition is provided, including one or more first probes and one or more second probes. Each of the first probe includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a first label, and a cleavage site for a first cleavage agent, wherein the first cleavage agent is capable of releasing the first label. Each of the second probes includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a second label, a quench moiety that renders the second label undetectable, and a cleavage site for the first cleavage agent. The first cleavage agent is capable of releasing the quench moiety, whereby the second label is rendered detectable.Type: ApplicationFiled: April 28, 2023Publication date: October 5, 2023Applicant: Ultivue, Inc.Inventors: Abdul Majeed Mohammed, Mael Manesse, Mack J. Schermer, Mark N. Bobrow, Michael Jules Natan
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Patent number: 11674178Abstract: Compositions, kits and methods for detecting a plurality of targets are provided herein. A probe-set composition is provided, including one or more first probes and one or more second probes. Each of the first probe includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a first label, and a cleavage site for a first cleavage agent, wherein the first cleavage agent is capable of releasing the first label. Each of the second probes includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a second label, a quench moiety that renders the second label undetectable, and a cleavage site for the first cleavage agent. The first cleavage agent is capable of releasing the quench moiety, whereby the second label is rendered detectable.Type: GrantFiled: December 13, 2019Date of Patent: June 13, 2023Assignee: Ultivue, Inc.Inventors: Abdul Majeed Mohammed, Mael Manesse, Mack J. Schermer, Mark N. Bobrow, Michael Jules Natan
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Publication number: 20200190583Abstract: Compositions, kits and methods for detecting a plurality of targets are provided herein. A probe-set composition is provided, including one or more first probes and one or more second probes. Each of the first probe includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a first label, and a cleavage site for a first cleavage agent, wherein the first cleavage agent is capable of releasing the first label. Each of the second probes includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a second label, a quench moiety that renders the second label undetectable, and a cleavage site for the first cleavage agent. The first cleavage agent is capable of releasing the quench moiety, whereby the second label is rendered detectable.Type: ApplicationFiled: December 13, 2019Publication date: June 18, 2020Inventors: Abdul Majeed Mohammed, Mael Manesse, Mack J. Schermer, Mark N. Bobrow, Michael Jules Natan
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Patent number: 8153367Abstract: The present invention concerns an array-based analytical system and method having an enhanced sensitivity which allows for simple and rapid analysis of relative unmodified samples which comprises an analytical system of the type having a plurality of different first members of a specific binding pair affixed in an array thereupon, a mixture including at least one second member of a specific binding pair capable of binding to one of the first members so as to form a specific binding pair which is affixed to the support member, and a reporter system that produces a detectable signal indicative of the presence of the specific binding pair on the support member and wherein the reporter system includes an amplified reporter system that is independent of layering.Type: GrantFiled: April 27, 2007Date of Patent: April 10, 2012Assignee: Perkinelmer Las, Inc.Inventors: Mark N. Bobrow, Karl E. Adler
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Publication number: 20110294227Abstract: The present invention relates to a method for determining the risk of a pregnant woman developing pre-eclampsia. The method comprises i) determining the level of one or more biochemical markers in a sample obtained from a pregnant woman, and ii) comparing the level of the at least one biochemical marker in the sample with the level of the same biochemical marker in a control sample. A difference in the level of the biochemical marker in the sample relative to the control sample is indicative of an increased risk of developing pre-eclampsia. The isoform biochemical markers are preferably P1GF-2 and P1GF-3. The present invention relates also to a method for determining whether a pregnant woman has pre-eclampsia and as well as a kit for assessing the risk or presence of pre-eclampsia. In addition, the invention relates also to a computer program used in these determinations.Type: ApplicationFiled: November 20, 2009Publication date: December 1, 2011Applicants: PERKINELMER HEALTH SCIENCES, INC., WALLAC OY, NTD LABORATORIES, INC.Inventors: Tarja Ahola, Heini Frang, Teemu Korpimäki, Pertti Hurskainen, Mark N. Bobrow, Jonathan B. Carmichael
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Patent number: 7291474Abstract: Disclosed are compounds of the general formulas: wherein Y is a moiety capable of being cleaved by a hydrolytic enzyme; L is a detectable label; X is a linking group; Z is a halogen; and R is hydrogen, an alkyl, or a halogen. Compounds of this general formula can be used as substrates in an analyte-dependent enzyme activation system, such as that employed in connection with catalyzed reporter depositions. Also disclosed are assays using the compounds, and kits for carrying out the assays.Type: GrantFiled: June 10, 2004Date of Patent: November 6, 2007Assignee: PerkinElmer LAS, Inc.Inventor: Mark N. Bobrow
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Patent number: 6066446Abstract: Disclosed is a member comprising an interactive material which is covalently bonded to a support body by a linker material. The member can be used as a part of an assay, and the support body may include a scintillator material.Type: GrantFiled: July 14, 1999Date of Patent: May 23, 2000Assignee: NEN Life Science Products, Inc.Inventors: Thomas R. Mullinax, Mark N. Bobrow, Michael E. Bembenek
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Patent number: 6030840Abstract: A method for the spectroscopic determination of a marker comprises:(a) contacting chelated lanthanide metal ions bound to a marker with a buffered solution comprising a detergent, an enhancer reagent and a polyanion, wherein the buffer maintains the pH of the solution within the range of about 3.5 to about 11.5 and the polyanion is present in sufficient concentration such that the lanthanide metal ion disassociates from the chelate complex and reassociates with the enhancer reagent, thereby transferring the lanthanide metal ion into fluorescent form; and(b) determining the amount of lanthanide metal ion liberated from the marker as a measure of the amount of marker present by subjecting the solution to a short radiation pulse and detecting the fluorescence of the lanthanide metal ion after the fluorescence from any background sources substantially has ceased.Type: GrantFiled: June 15, 1998Date of Patent: February 29, 2000Assignee: NEN Life Sciences, Inc.Inventors: Thomas Robert Mullinax, Margaret R. Cody, Mark N. Bobrow
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Patent number: 5972595Abstract: This invention relates to a hydrophobic layer formed on a solid support, the hydrophobic layer incorporating by hydrophobic interactions an amphipathic enzyme substrate labeled with a reporter on its hydrophilic region, and the use of the same to conduct various enzyme assays without extraction steps.Type: GrantFiled: December 16, 1998Date of Patent: October 26, 1999Assignee: NEN Life Science Products, Inc.Inventors: Patricia Kasila, Thomas R. Mullinax, Michael E. Bembenek, Mark N. Bobrow
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Patent number: 5583001Abstract: The present invention concerns a method to catalyze reporter deposition to improve detection or quantitation of an analyte in a sample by amplifying the detector signal which comprises reacting an analyte dependent enzyme activation system with a conjugate consisting of a detectably labeled substrate specific for the enzyme system, said conjugate reacts with the analyte dependent enzyme activation system to form an activated conjugate which deposits substantially wherever receptor for the activated conjugate is immobilized, said receptor not being reactive with the analyte dependent enzyme activation system.In another embodiment the invention concerns an assay for detecting or quantitating the presence or absence of an analyte in a sample using catalyzed reporter deposition to amplify the reporter signal.Type: GrantFiled: May 4, 1994Date of Patent: December 10, 1996Assignee: E. I. du Pont de Nemours and CompanyInventors: Mark N. Bobrow, Gerald J. Litt
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Patent number: 5391479Abstract: A method for determining total analyte concentration in a sample having both free and bound analyte is described. This method involves (a) disassociating immune complexes, (b) assaying for the concentration of free analyte, (c) assaying at least once for the concentration of free analyte in the sample wherein said sample contains a known quantity of analyte which has been added to the sample, and (d) determining total analyte concentration from the assayed concentrations obtained in steps (b) and (c).Type: GrantFiled: October 29, 1992Date of Patent: February 21, 1995Assignee: E. I. Du Pont de Nemours and CompanyInventor: Mark N. Bobrow
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Patent number: 5238817Abstract: A substrate for detecting peroxidase activity in a peroxidase-based assay which includes a substantially aqueous solution of at least a 1-naphthol derivative and tetramethylbenzidine is described.Type: GrantFiled: July 12, 1991Date of Patent: August 24, 1993Assignee: E. I. Du Pont de Nemours and CompanyInventors: Mark N. Bobrow, Gerald J. Litt
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Patent number: 5196306Abstract: The present invention concerns a method to catalyze reporter deposition to improve detection or quantitation of an analyte in a sample by amplifying the detector signal which comprises reacting an analyte dependent enzyme activation system with a conjugate consisting of a detectably labeled substrate specific for the enzyme system, said conjugate reacts with the analyte dependent enzyme activation system to form an activated conjugate which deposits substantially wherever receptor for the activated conjugate is immobilized, said receptor not being reactive with the analyte dependent enzyme activation system. In another embodiment the invention concerns an assay for detecting or quantitating the presence or absence of an analyte in a sample using catalyzed reporter deposition to amplify the reporter signal.Type: GrantFiled: September 28, 1990Date of Patent: March 23, 1993Assignee: E. I. Du Pont de Nemours and CompanyInventors: Mark N. Bobrow, Gerald J. Litt