Abstract: The present invention relates to a method for producing haploid, dihaploid, polyhaploid and/or doubled haploid plants of the family Cucurbitaceae from isolated microspores, wherein said method comprises a) culturing isolated microspores to obtain embryos competent for plant regeneration, wherein the microspores have been isolated from plant material of a donor plant of the family Cucurbitaceae; and b) regenerating plants from the embryos; wherein step (a) comprises contacting the microspores with one or more inhibitor of histone deacetylase (HDACi) and one or more polyamine. The present invention also relates to a method for producing haploid, dihaploid, polyhaploid and/or doubled haploid embryos, to related kits and compositions, and to plants obtained according to the methods.

Abstract: The present invention relates to a method for producing haploid, dihaploid, polyhaploid and/or doubled haploid plants of the family Cucurbitaceae from isolated microspores, wherein said method comprises a) culturing isolated microspores to obtain embryos competent for plant regeneration, wherein the microspores have been isolated from plant material of a donor plant of the family Cucurbitaceae; and b) regenerating plants from the embryos; wherein step (a) comprises contacting the microspores with one or more inhibitor of histone deacetylase (HDACi) and one or more polyamine. The present invention also relates to a method for producing haploid, dihaploid, polyhaploid and/or doubled haploid embryos, to related kits and compositions, and to plants obtained according to the methods.

Abstract: A MOSFET, an op-amp, a comparator circuit, and voltage dividers with capacitors are employed in combination to effectuate a soft-start switch with current limiting. The transconductance of the MOSFET is employed so that no sense resistor is required. The MOSFET and op-amp are configured as a closed-loop feedback circuit in which the output of the op-amp is coupled to the gate of the MOSFET and the inverting input of the op-amp is coupled to the output of the soft-start switch via a voltage divider. A first RC circuit provides a voltage to the non-inverting input of the op-amp which can be triggered to gradually rise from a value close to zero to some reference voltage so as to soft-start a load. Current limiting means are effectuated by a comparator circuit and voltage dividers with capacitors.