Abstract: The present disclosure relates to methods, systems, and kits for analysis of macromolecule analytes such as polypeptides by employing a set of multi-component detection agents. In some embodiments, the method is useful for identifying a partial sequence, or a terminal amino acid of a polypeptide analyte in a high-throughput manner. In some embodiments, the multi-component detection agent includes an RNAP portion A and an RNAP portion B which, when in proximity, can generate a detectable signal, such as specific RNA transcripts.

Abstract: A method of cleaning particulate coal which is fed with a dense medium slurry as an inlet feed to a cyclone separator. The coal particle size distribution is in the range of from about 37 microns to about 600 microns. The dense medium comprises water and ferromagnetic particles that have a relative density in the range of from about 4.0 to about 7.0. The ferromagnetic particles of the dense medium have particle sizes of less than about 15 microns and at least a majority of the particle sizes are less than about 5 microns. In the cyclone, the particulate coal and dense-medium slurry is separated into a low gravity product stream and a high gravity produce stream wherein the differential in relative density between the two streams is not greater than about 0.2. The low gravity and high gravity streams are treated to recover the ferromagnetic particles therefrom.